Stern J A, Lui R C, LaRegina M C, Herbold D R, Tolman K C, Johnson F E
Department of Surgery, St. Louis University Medical Center, Missouri 63110-0250.
J Androl. 1990 Jul-Aug;11(4):390-5.
Male germ cells are quite sensitive to interruption in blood flow. Eight weeks after subjection to 45 minutes of testicular ischemia, the spermatogenic epithelium of the rat remains significantly damaged, though other cell types are well preserved. The authors evaluated the testicular recovery of the rats at 8 and 72 weeks after the 45-minute period of warm ischemia. Twenty-eight rats were studied: 14 underwent 45 minutes of total left testicular ischemia; 14 received no treatment. Four rats from each group were necropsied at 8 weeks to document the ischemic injury. At 72 weeks, the 18 surviving rats were necropsied to evaluate the long-term outcome of the treatment. At 8 weeks, significant left testicular injury was documented. However, at 72 weeks there was no difference in testicular weight or sperm head count between the groups: in all 36 testicles, the repopulation index was 1.00, the epididymal index was 3+, the modified Johnsen index was 14, and the spermatic cord score was 7 (all are maximum obtainable scores). Neither contralateral orchiopathy nor injury to spermatic cord structures was observed. Our work shows that ischemia-induced testicular injury is fully reversible with time in this model.
雄性生殖细胞对血流中断相当敏感。在经历45分钟的睾丸缺血后八周，大鼠的生精上皮仍有明显损伤，尽管其他细胞类型保存完好。作者评估了在45分钟的热缺血期后8周和72周时大鼠睾丸的恢复情况。研究了28只大鼠：14只经历了45分钟的左侧睾丸完全缺血；14只未接受治疗。每组4只大鼠在8周时进行尸检以记录缺血损伤。在72周时，对18只存活的大鼠进行尸检以评估治疗的长期结果。在8周时，记录到左侧睾丸有明显损伤。然而，在72周时，两组之间的睾丸重量或精子头计数没有差异：在所有36个睾丸中，再殖指数为1.00，附睾指数为3+，改良约翰森指数为14，精索评分7分（均为可获得的最高分）。未观察到对侧睾丸病或精索结构损伤。我们的研究表明，在该模型中，缺血诱导的睾丸损伤随时间推移是完全可逆的。
