Kostner G M, Avogaro P, Bon G B, Cazzolato G, Quinci G B
Clin Chem. 1979 Jun;25(6):939-42.
Factors reflecting the concentration of high-density lipoproteins in serum were assessed for 108 men and 106 women participating in a Venetian screening program for hyperlipoproteinemia. The methods applied, optimized in our laboratory, were: (a) cholesterol in high-density lipoproteins, determined in the supernate after sedimentation of the very-low-density lipoproteins + low-density lipoproteins with dextran sulfate or sodium phosphotungstate; and (b) immunochemical quantitation of apolipoprotein A-I and apolipoprotein A-II by Laurell's "rocket" technique. The latter determinations were performed with total serum before and after delipidation with diispropyl ether/n-butanol (6/4 by vol). The dextran sulfate method gave about 5% higher values than did the phosphotungstate method, but the correlation between the two was excellent (r = 0.95). Results of the immunochemical quantitation indicate that delipidation of lipoproteins before Laurell electrophoresis may not be necessary if only freshly drawn sera are used.
对参与威尼斯高脂血症筛查项目的108名男性和106名女性，评估了反映血清中高密度脂蛋白浓度的各项因素。我们实验室优化应用的方法如下：(a) 高密度脂蛋白中的胆固醇，在使用硫酸葡聚糖或磷钨酸钠沉淀极低密度脂蛋白 + 低密度脂蛋白后的上清液中测定；(b) 采用劳雷尔“火箭”技术对载脂蛋白A-I和载脂蛋白A-II进行免疫化学定量。后一项测定是在用二异丙醚/正丁醇（体积比6/4）脱脂前后的全血清中进行的。硫酸葡聚糖法测得的值比磷钨酸盐法高约5%，但两者之间的相关性极佳（r = 0.95）。免疫化学定量结果表明，如果仅使用新鲜采集的血清，在劳雷尔电泳前对脂蛋白进行脱脂可能没有必要。
