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超声合成技术制备 H6N2 禽流感病毒 pHEMA 纳米聚合物疫苗的初步研究。

Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation.

机构信息

Murdoch Applied Nanotechnology Research Group, Murdoch University, Murdoch, WA, Australia.

出版信息

Int J Nanomedicine. 2011;6:2167-74. doi: 10.2147/IJN.S24272. Epub 2011 Sep 28.

DOI:10.2147/IJN.S24272
PMID:22114480
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3215157/
Abstract

This preliminary study investigated the use of poly (2-hydroxyethyl methacrylate) (pHEMA) nanoparticles for the delivery of the deoxyribonucleic acid (DNA) vaccine pCAG-HAk, which expresses the full length hemagglutinin (HA) gene of the avian influenza A/Eurasian coot/Western Australian/2727/1979 (H6N2) virus with a Kozak sequence which is in the form of a pCAGGS vector. The loaded and unloaded nanoparticles were characterized using field-emission scanning electron microscopy. Further characterizations of the nanoparticles were made using atomic force microscopy and dynamic light scattering, which was used to investigate particle size distributions. This preliminary study suggests that using 100 μg of pHEMA nanoparticles as a nanocarrier/adjuvant produced a reduction in virus shedding and improved the immune response to the DNA vaccine pCAG-HAk.

摘要

本初步研究探讨了聚（2-羟乙基甲基丙烯酸酯）（pHEMA）纳米粒子在递送表达禽甲型流感 A/欧亚水禽/西澳大利亚/2727/1979（H6N2）病毒全长血凝素（HA）基因的脱氧核糖核酸（DNA）疫苗 pCAG-HAk 中的应用，该基因带有 Kozak 序列，形式为 pCAGGS 载体。使用场发射扫描电子显微镜对负载和未负载的纳米粒子进行了表征。进一步使用原子力显微镜和动态光散射对纳米粒子进行了表征，用于研究粒径分布。本初步研究表明，使用 100 μg pHEMA 纳米粒子作为纳米载体/佐剂可减少病毒脱落并提高对 DNA 疫苗 pCAG-HAk 的免疫应答。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38d/3215157/f636d817897e/ijn-6-2167f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38d/3215157/10d23109e29f/ijn-6-2167f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38d/3215157/19583d7c852a/ijn-6-2167f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38d/3215157/f636d817897e/ijn-6-2167f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38d/3215157/10d23109e29f/ijn-6-2167f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38d/3215157/19583d7c852a/ijn-6-2167f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a38d/3215157/f636d817897e/ijn-6-2167f3.jpg

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2
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3
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4
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