Division of Public Health Services, New Hampshire Department of Health and Human Services, Concord, NH 03301, USA.
Influenza Other Respir Viruses. 2012 May;6(3):e30-4. doi: 10.1111/j.1750-2659.2011.00313.x. Epub 2011 Nov 24.
The performance of rapid influenza diagnostic tests (RIDTs) in detecting influenza A(H1N1) 2009 has varied widely. Evaluations of RIDTs among infected individuals across all age groups have not been described in depth.
Determine RIDT clinical sensitivity in comparison with influenza detection using real-time RT-PCR among patients infected with influenza A(H1N1) 2009 across all age groups.
This study analyzed respiratory specimens received by the New Hampshire Public Health Laboratories (NHPHL) from September 1, 2009, through December 31, 2009. RIDT performance was evaluated among different age groups of patients determined to be infected with influenza A (H1N1) 2009, and the association between age and RIDT sensitivity was determined.
Of 1373 specimens examined, 269 tested positive for influenza A(H1N1) 2009 by real-time RT-PCR (rRT-PCR) and had RIDT results available. Overall clinical sensitivity and specificity of RIDTs were 53·9 and 98·5%, respectively. By age group, clinical sensitivity was 85·7% in patients <2 years old, 60·3% in patients between 2- and 39 years old, and 33·3% in patients aged 40 and older. Logistic regression analysis indicated that increasing age was negatively associated with RIDT performance.
Rapid influenza diagnostic test sensitivity decreased significantly with increasing age. Findings from this study may impact a clinician's interpretation of RIDT test results and ultimately have implications in clinical decision-making.
快速流感诊断检测（RIDT）在检测甲型 H1N1 2009 流感方面的表现差异很大。尚未深入描述对所有年龄段感染者的 RIDT 评估。
确定 RIDT 与实时 RT-PCR 相比在检测所有年龄段甲型 H1N1 2009 流感感染者中的临床灵敏度。
本研究分析了 2009 年 9 月 1 日至 2009 年 12 月 31 日期间新罕布什尔州公共卫生实验室（NHPHL）收到的呼吸道标本。评估了 RIDT 在确定为感染甲型流感（H1N1）2009 的不同年龄组患者中的性能，并确定了年龄与 RIDT 敏感性之间的关系。
在检查的 1373 个标本中，有 269 个通过实时 RT-PCR（rRT-PCR）检测为甲型 H1N1 2009 阳性，并可获得 RIDT 结果。RIDT 的总体临床灵敏度和特异性分别为 53.9%和 98.5%。按年龄组，<2 岁的患者临床灵敏度为 85.7%，2-39 岁的患者为 60.3%，40 岁及以上的患者为 33.3%。逻辑回归分析表明，年龄的增加与 RIDT 性能呈负相关。
快速流感诊断检测的灵敏度随着年龄的增加而显著下降。本研究的结果可能会影响临床医生对 RIDT 检测结果的解释，最终对临床决策产生影响。
