Wu Qi, Song Yong-Chun, Xu Hao, Guo Ye, Li Jing, Tan Ren-Xiang
Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
J Asian Nat Prod Res. 2011 Dec;13(12):1110-21. doi: 10.1080/10286020.2011.618451.
This paper describes improved optimization method that combines the one-factor-at-a-time method (OFAT), Plackett-Burman design, and the response surface method (RSM), which were used to optimize the medium for the production of fumigaclavine C (FC) and helvolic acid (HA) from endophytic Aspergillus fumigatus CY018 simultaneously. The ideal carbon and nitrogen sources for the two compounds were assessed initially via the one-factor-at-a-time method. Three key cultivation factors (pH, phosphate, and inoculum size) were chosen based on the results of Plackett-Burman design, and subsequently optimized by the central composite design. The two metabolites were amply afforded when the cultivation was carried out with the inoculum size of 2.45% at pH 4.2 and 28°C for 19 days in the medium containing (g/l): mannitol 50, sodium succinate 5.4, NaNO₃ 2, MgSO₄·7H₂O 0.3, FeSO₄·7H₂O 0.01, and KH₂PO₄ 0.67. The highest yields of FC and HA achieved herein were 17.26 and 16.88 mg/l. This work might be the first endeavor leading to the improved simultaneous production of two complex active metabolites with a single strain.
本文描述了一种改进的优化方法，该方法结合了单因素法（OFAT）、Plackett-Burman设计和响应面法（RSM），用于同时优化内生烟曲霉CY018生产烟曲霉黄质C（FC）和蜂窝酸（HA）的培养基。首先通过单因素法评估了这两种化合物的理想碳源和氮源。基于Plackett-Burman设计的结果选择了三个关键培养因素（pH、磷酸盐和接种量），随后通过中心复合设计进行优化。在含有（g/l）：甘露醇50、琥珀酸钠5.4、NaNO₃ 2、MgSO₄·7H₂O 0.3、FeSO₄·7H₂O 0.01和KH₂PO₄ 0.67的培养基中，于pH 4.2、28°C下以2.45%的接种量培养19天时，这两种代谢产物得到了大量产生。本文中FC和HA的最高产量分别为17.26和16.88 mg/l。这项工作可能是首次尝试利用单一菌株提高两种复杂活性代谢产物的同时产量。
