• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用响应面法技术生产新型麦角生物碱。

Novel Ergot Alkaloids Production from Employing Response Surface Methodology Technique.

机构信息

Department of Botany, GC University, Lahore 54000, Pakistan.

Food and Biotechnology Research Center, PCSIR Laboratories Complex, Lahore 54000, Pakistan.

出版信息

Toxins (Basel). 2020 Jun 29;12(7):427. doi: 10.3390/toxins12070427.

DOI:10.3390/toxins12070427
PMID:32610508
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7405006/
Abstract

Ergot alkaloids are novel pharmaceutical and therapeutic agents synthesized in this study using fungal species To get the maximum yield of ergot alkaloids a statistical process of response surface methodology was employed using surface culture fermentation technique. Initially, the strain of was improved using physical (ultraviolet (UV) and chemical (ethyl methane sulfonate (EMS) treatments to get the maximum yield of ergot alkaloids through surface culture fermentation technique. After improving the strain, survival rate of colonies of treated with UV and EMS was observed. Only 2.04% living colonies were observed after 150 min of exposure of in UV light and 3.2% living colonies were observed after 20 min of the exposure in EMS. The mutated strains of were screened for their production of ergot alkaloids and after fermentation experiments, maximum yield was obtained from PCUV-4 and PCEMS-1 strains. After strain improvement, Plackett-Burman design (PBD) and Box-Behnken design (BBD) of RSM were employed and 10-fold yield enhancement (35.60 mg/100 mL) of ergot alkaloids was achieved. This enhancement in yield of ergot alkaloids proved the positive impacts of RSM and UV on the yield of ergot alkaloids. The study provides a cost effective, economical and sustainable process to produce medically important ergot alkaloids which can be used in various pharmaceutical formulations to treat human diseases.

摘要

麦角生物碱是本研究中合成的新型药物和治疗剂，使用真菌物种得到。为了获得最大的麦角生物碱产量，采用表面培养发酵技术，利用响应面法进行了统计过程。最初，通过物理（紫外线（UV）和化学（乙基甲烷磺酸酯（EMS）处理来改善 的菌株，以通过表面培养发酵技术获得最大的麦角生物碱产量。在改良菌株后，观察到用 UV 和 EMS 处理的 菌落的存活率。在 UV 光下暴露 150 分钟后，仅观察到 2.04%的存活菌落，在 EMS 中暴露 20 分钟后，观察到 3.2%的存活菌落。筛选出产生麦角生物碱的突变菌株，并经过发酵实验，从 PCUV-4 和 PCEMS-1 菌株中获得最大产量。在菌株改良后，采用 Plackett-Burman 设计（PBD）和响应面法的 Box-Behnken 设计（BBD）进行了实验，麦角生物碱的产量提高了 10 倍（35.60 mg/100 mL）。麦角生物碱产量的提高证明了 RSM 和 UV 对麦角生物碱产量的积极影响。该研究提供了一种具有成本效益、经济和可持续的生产方法，可以生产具有重要医学价值的麦角生物碱，可用于各种药物制剂以治疗人类疾病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/b9b21409ba83/toxins-12-00427-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/c22bc36d8e93/toxins-12-00427-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/03c0d4770085/toxins-12-00427-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/c92fb03918e4/toxins-12-00427-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/71b3eeb4d05e/toxins-12-00427-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/ac251150d516/toxins-12-00427-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/92a6c0751d69/toxins-12-00427-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/404c9d93bd0f/toxins-12-00427-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/b9b21409ba83/toxins-12-00427-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/c22bc36d8e93/toxins-12-00427-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/03c0d4770085/toxins-12-00427-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/c92fb03918e4/toxins-12-00427-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/71b3eeb4d05e/toxins-12-00427-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/ac251150d516/toxins-12-00427-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/92a6c0751d69/toxins-12-00427-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/404c9d93bd0f/toxins-12-00427-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4de/7405006/b9b21409ba83/toxins-12-00427-g008.jpg

相似文献

1
Novel Ergot Alkaloids Production from Employing Response Surface Methodology Technique.利用响应面法技术生产新型麦角生物碱。
Toxins (Basel). 2020 Jun 29;12(7):427. doi: 10.3390/toxins12070427.
2
Screening and optimization of some inorganic salts for the production of ergot alkaloids from Penicillium species using surface culture fermentation process.利用表面培养发酵工艺从青霉菌种生产麦角生物碱的一些无机盐的筛选与优化
Pak J Pharm Sci. 2016 Mar;29(2):407-14.
3
[Fungus Penicillium citrinum, isolated from permafrost sediments, as a producer of ergot alkaloids and new quinoline alkaloids quinocitrinines].[从永久冻土沉积物中分离出的青霉属真菌，作为麦角生物碱和新喹啉生物碱喹诺柠檬碱的生产者]
Prikl Biokhim Mikrobiol. 2005 Sep-Oct;41(5):568-72.
4
[The effect of some amino acids on the growth and biosynthesis of ergot alkaloids and quinocitrinins in the fungus Penicillium citrinum Thom 1910].[某些氨基酸对1910年汤姆氏桔青霉中麦角生物碱和喹诺菌素生长及生物合成的影响]
Mikrobiologiia. 2006 May-Jun;75(3):334-41.
5
[Transport of ergot alkaloids and quinocitrinins in the producing fungus Penicillium citrinum].[产黄青霉中麦角生物碱和醌型桔霉素的转运]
Mikrobiologiia. 2007 Jul-Aug;76(4):456-61.
6
[Effect on microelements on biosynthes of secondary metabolites in the fungus Penicillium citrinum Thom VKM F-1079].[微量元素对桔青霉VKM F-1079次生代谢产物生物合成的影响]
Mikrobiologiia. 2000 Sep-Oct;69(5):642-6.
7
Ergot Alkaloid Synthesis Capacity of Penicillium camemberti.卡门柏青霉的麦角生物碱合成能力。
Appl Environ Microbiol. 2018 Sep 17;84(19). doi: 10.1128/AEM.01583-18. Print 2018 Oct 1.
8
[The influence of medium composition on alkaloid biosynthesis by Penicillium citrinum].[培养基成分对桔青霉生物碱生物合成的影响]
Prikl Biokhim Mikrobiol. 2010 Sep-Oct;46(5):572-6.
9
Statistical optimization of alkaline protease production from Penicillium citrinum YL-1 under solid-state fermentation.固态发酵条件下产黄青霉YL-1碱性蛋白酶产量的统计优化
Prep Biochem Biotechnol. 2015;45(5):447-62. doi: 10.1080/10826068.2014.923450.
10
Production, detection, and purification of clavine-type ergot alkaloids.棒麦角碱型麦角生物碱的生产、检测与纯化。
Methods Mol Biol. 2012;944:121-31. doi: 10.1007/978-1-62703-122-6_8.

引用本文的文献

1
Regulation and induction of fungal secondary metabolites: a comprehensive review.真菌次生代谢产物的调控与诱导：综述
Arch Microbiol. 2025 Jul 1;207(8):189. doi: 10.1007/s00203-025-04386-0.
2
Ergot alkaloid control in biotechnological processes and pharmaceuticals (a mini review).生物技术过程和药物中的麦角生物碱控制（一篇小型综述）
Front Toxicol. 2024 Oct 8;6:1463758. doi: 10.3389/ftox.2024.1463758. eCollection 2024.
3
An overview of mycotoxicoses in rabbits.兔真菌毒素中毒概述。

本文引用的文献

1
Screening and optimization of some inorganic salts for the production of ergot alkaloids from Penicillium species using surface culture fermentation process.利用表面培养发酵工艺从青霉菌种生产麦角生物碱的一些无机盐的筛选与优化
Pak J Pharm Sci. 2016 Mar;29(2):407-14.
2
Biosynthetic pathways of ergot alkaloids.麦角生物碱的生物合成途径。
Toxins (Basel). 2014 Dec 10;6(12):3281-95. doi: 10.3390/toxins6123281.
3
Partial reconstruction of the ergot alkaloid pathway by heterologous gene expression in Aspergillus nidulans.在构巢曲霉中通过异源基因表达部分重建麦角生物碱途径。
J Vet Diagn Invest. 2024 Sep;36(5):638-654. doi: 10.1177/10406387241255945. Epub 2024 May 28.
4
Global Impact of Ergot Alkaloids.麦角生物碱的全球影响。
Toxins (Basel). 2022 Mar 3;14(3):186. doi: 10.3390/toxins14030186.
Toxins (Basel). 2013 Feb 22;5(2):445-55. doi: 10.3390/toxins5020445.
4
Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology.响应面法优化紫色链霉菌深层发酵产碱性木聚糖酶。
Indian J Microbiol. 2007 Jun;47(2):144-52. doi: 10.1007/s12088-007-0028-4. Epub 2007 Jul 8.
5
Medium optimization for enhanced co-production of two bioactive metabolites in the same fermentation by a statistical approach.通过统计方法优化培养基以提高同一发酵过程中两种生物活性代谢产物的联产
J Asian Nat Prod Res. 2011 Dec;13(12):1110-21. doi: 10.1080/10286020.2011.618451.
6
Bioactive alkaloids produced by fungi. I. Updates on alkaloids from the species of the genera Boletus, Fusarium and psilocybe.真菌产生的生物活性生物碱。I. 牛肝菌属、镰刀菌属和裸盖菇属物种生物碱的最新研究进展。
Pak J Pharm Sci. 2010 Jul;23(3):349-57.
7
Application of response surface methodology in medium optimization for protease production by the new strain of Serratia marcescens SB08.响应面法在粘质沙雷氏菌新菌株SB08产蛋白酶培养基优化中的应用
Pol J Microbiol. 2009;58(2):117-24.
8
Cyclic resolution of racemic ibuprofen via coupled efficient lipase and acid-base catalysis.通过高效脂肪酶与酸碱催化偶联实现外消旋布洛芬的循环拆分
Chirality. 2009 Mar;21(3):349-53. doi: 10.1002/chir.20578.
9
Claviceps cyperi, a new cause of severe ergotism in dairy cattle consuming maize silage and teff hay contaminated with ergotised Cyperus esculentus (nut sedge) on the Highveld of South Africa.麦角菌（Claviceps cyperi）是南非高原地区奶牛食用被麦角化的香附子（莎草）污染的玉米青贮饲料和画眉草干草后发生严重麦角中毒的一个新病因。
Onderstepoort J Vet Res. 2005 Mar;72(1):23-37. doi: 10.4102/ojvr.v72i1.221.
10
Selection of medium components by Plackett-Burman design for production of L(+) lactic acid by Lactobacillus amylophilus GV6 in SSF using wheat bran.利用Plackett-Burman设计法选择培养基成分，用于嗜淀粉乳杆菌GV6在固态发酵中以麦麸为原料生产L(+)乳酸。
Bioresour Technol. 2005 Mar;96(4):485-90. doi: 10.1016/j.biortech.2004.05.020.