a School of Microbiology and Immunology , The University of New South Wales , Sydney , 2052 , Australia Fax: E-mail:
Biofouling. 2000;15(1-3):109-17. doi: 10.1080/08927010009386302.
A range of bacteria, including the marine bacterium Pseudoalteromonas tunicata which produces antifouling compounds, and Escherichia coli were used to investigate methods for immobilising bacteria in gels. Different types of matrices were screened using the survival of barnacle nauplii as a bioassay. A Dupont® polyvinylalcohol (PVOH) 10% gel was found to be the optimal matrix. This non-toxic gel remained stable in seawater while allowing for an outflux of active biological compounds from the bacterial cells. The presence of active bacterial cells in the matrix was tested by CTC-staining, green fluorescent protein (GFP) expressing bacteria and a barnacle larvae bioassay. The Dupont® PVOH 10% gels containing P. tunicata cells were inhibitory against larvae for a period of up to 2 weeks. In further studies using gels containing immobilised bacteria, the E. coli strain C600 was employed based on its cell size, stress resistance and the fact that a plasmid for the expression of GFP could be transferred and maintained in the cells. Immobilised E. Coli cells maintained their viability in the Dupont® PVOH 10% gels for as long as 2 months, and the life-span of these "biologically active"; gels was increased to more than 2 months by the incorporation of small beads into the gels. The results indicate that bacteria can be immobilised in coatings for periods of time consistent with the needs of some antifouling and antibacterial applications.
一系列细菌，包括产生防污化合物的海洋细菌 Pseudoalteromonas tunicata 和 Escherichia coli，被用于研究将细菌固定在凝胶中的方法。使用藤壶幼体的存活作为生物测定筛选了不同类型的基质。发现杜邦®聚乙烯醇 (PVOH) 10%凝胶是最佳基质。这种无毒凝胶在海水中保持稳定，同时允许细菌细胞中的活性生物化合物流出。通过 CTC 染色、表达绿色荧光蛋白 (GFP) 的细菌和藤壶幼虫生物测定来测试基质中活性细菌细胞的存在。含有 P. tunicata 细胞的杜邦®PVOH 10%凝胶对幼虫具有长达 2 周的抑制作用。在使用固定化细菌的进一步研究中，根据其细胞大小、抗应激能力以及 GFP 表达质粒可以转移和维持在细胞中的事实，选择了大肠杆菌菌株 C600。固定化大肠杆菌细胞在杜邦®PVOH 10%凝胶中保持活力长达 2 个月，通过在凝胶中加入小珠，这些“生物活性”凝胶的寿命延长至 2 个月以上。研究结果表明，细菌可以在涂层中固定一段时间，这与一些防污和抗菌应用的需求一致。
