辅助生殖小鼠模型中胎盘和胎肝 Ugt1a 基因的上调。
Upregulation of Ugt1a genes in placentas and fetal livers in a murine model of assisted reproduction.
机构信息
Dept. Tropical Medicine, Medical Microbiology and Pharmacology, University of Hawaii at Mānoa, Honolulu, HI 96813, United States.
出版信息
Placenta. 2012 Jan;33(1):77-80. doi: 10.1016/j.placenta.2011.11.002. Epub 2011 Nov 25.
Abstract
Genes from Ugt1a family in placenta and fetal liver are responsible for hormone, nutrient and chemical balance during pregnancy. Assisted reproduction technologies (ART) i.e. intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) alter steroid homeostasis in pregnancy through increased glucuronidation. Here we show that ART (particularly ICSI) upregulates Ugt1a1, 1a2, 1a6 and 1a9 expression in murine placentas and fetal livers with higher mRNA related to lower progesterone (1a1) and cholesterol (1a2, 1a6) in placentas. Greater steroid clearance in ART through transcriptional upregulation of Ugt1a in the placental-fetal unit may decrease the availability of essential molecules, mediating negative reproductive outcomes.
摘要
胎盘和胎肝中的 Ugt1a 家族基因负责妊娠期间激素、营养和化学平衡。辅助生殖技术（ART），即胞浆内单精子注射（ICSI）和体外受精（IVF），通过增加葡萄糖醛酸化来改变妊娠期间的类固醇动态平衡。在这里，我们表明 ART（特别是 ICSI）会上调小鼠胎盘和胎肝中的 Ugt1a1、1a2、1a6 和 1a9 的表达，与胎盘中的较低孕激素（1a1）和胆固醇（1a2、1a6）相关的 mRNA 更高。通过在胎盘-胎儿单位中转录上调 Ugt1a，ART 中更大的类固醇清除可能会降低必需分子的可用性，从而导致不良的生殖结局。
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3
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