Viral Respiratory Pathogens Research Unit and the Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.
J Clin Microbiol. 2012 Feb;50(2):506-8. doi: 10.1128/JCM.05999-11. Epub 2011 Nov 23.
We evaluated the prevalence of respiratory virus infection (RVI) in 403 illnesses of 364 persons hospitalized over a 2-year period with acute respiratory conditions using virus-specific reverse transcription-PCR (RT-PCR) assays in addition to cell culture and serology. RVIs were identified in >75% of children under 5 years of age and 25 to 37% of adults. The molecular assays doubled the number of infections identified; picornaviruses were the most frequent in patients of all ages, followed by respiratory syncytial virus and influenza viruses.
我们使用病毒特异性逆转录聚合酶链反应(RT-PCR)检测法,对 364 名患有急性呼吸道疾病住院患者的 403 例疾病进行检测,以评估呼吸道病毒感染(RVI)的流行情况,此外还进行了细胞培养和血清学检测。在 5 岁以下的儿童中,超过 75%的人以及 25 岁至 37%的成年人患有 RVI。分子检测法使感染数量增加了一倍;在所有年龄段的患者中,肠道病毒最为常见,其次是呼吸道合胞病毒和流感病毒。