Díaz Patricia V, Valdivia Gonzalo, Gaggero Aldo A, Bono M R, Zepeda Guillermo, Rivas Mabel, Uasapud Paola, Pinto Ricardo A, Boza M Lina, Guerrero Julia
From the Institute of Biomedical Sciences, Faculty of Medicine, University of Chile (PVD, AAG, JG); Department of Public Health, Catholic University (GV); Department of Immunology, Faculty of Sciences, University of Chile (MRB); Department of Pediatrics, Faculty of Medicine, University of Chile (GZ); San Borja-Arriarán, Clinical Hospital (MR, MLB); and Family Health Center "Agustin Cruz Melo", North Health Service, Santiago (PU).
Medicine (Baltimore). 2015 Sep;94(39):e1512. doi: 10.1097/MD.0000000000001512.
Respiratory syncytial virus (RSV) and human rhinovirus (HRV) respiratory infection in children induce production of inflammatory interleukins (ILs) in the respiratory epithelium. As IL(s) determine the severity of illness, the purpose of this study was to identify the pro-inflammatory IL(s) that could be predictor(s) of clinical severity. One hundred and fifteen patients <2 years old with bronchiolitis due to RSV and /or HRV and 38 controls were selected from a hospital and an outpatient clinic. Clinical data of all patients were recorded. Severity was defined by the number of days with oxygen need. Nasopharyngeal aspirates (NPA) were collected to perform viral diagnosis by quantitative reverse transcription and polymerase chain reaction (qRT-PCR) and to quantify ILs: TNF-α, IL-10, IL-6, IL-1β, and IL-8, by flow cytometry. Simple and multiple regression and receiver operating characteristic (ROC) curves were used for statistical analysis. Of the patients selected 60 were single RSV, 28 RSV associated to HRV, and 27 single HRV. All patients (115) showed significantly higher IL levels when compared with controls. Levels of IL-6, IL-1β, and IL-8 detected in NPA from RSV single and associated to HRV were significantly higher than HRV infected and positively associated with days requiring O2.Levels of IL-6, IL-1β, and IL-8 detected in NPA from patients infected with RSV only or with both RSV and HRV are increased, and any of those 3 cytokines may have a predictive value for the number of days with need of supplemental oxygen.
呼吸道合胞病毒（RSV）和人鼻病毒（HRV）引起的儿童呼吸道感染可诱导呼吸道上皮产生炎性白细胞介素（ILs）。由于ILs决定疾病的严重程度，本研究的目的是确定可能作为临床严重程度预测指标的促炎ILs。从一家医院和一家门诊诊所选取了115名2岁以下因RSV和/或HRV引起细支气管炎的患者以及38名对照。记录了所有患者的临床数据。严重程度根据需要吸氧的天数来定义。收集鼻咽抽吸物（NPA），通过定量逆转录聚合酶链反应（qRT-PCR）进行病毒诊断，并通过流式细胞术定量检测ILs：肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和白细胞介素-8（IL-8）。采用简单和多元回归以及受试者工作特征（ROC）曲线进行统计分析。在所选患者中，60例为单纯RSV感染，28例为RSV合并HRV感染，27例为单纯HRV感染。与对照组相比，所有115例患者的IL水平均显著升高。在单纯RSV感染及RSV合并HRV感染患者的NPA中检测到的IL-6、IL-1β和IL-8水平显著高于HRV感染患者，且与需要吸氧的天数呈正相关。仅感染RSV或同时感染RSV和HRV的患者NPA中检测到的IL-6、IL-1β和IL-8水平升高，这三种细胞因子中的任何一种都可能对需要补充氧气的天数具有预测价值。
