鸡碳酸酐酶同工酶-III（CA-III）的纯化及其在白来航鸡中的测定。

Purification of chicken carbonic anhydrase isozyme-III (CA-III) and its measurement in White Leghorn chickens.

机构信息

Laboratories of Veterinary Physiology, School of Veterinary Medicine, Azabu, University, Sagamihara, Kanagawa, Japan.

出版信息

Acta Vet Scand. 2011 Nov 26;53(1):63. doi: 10.1186/1751-0147-53-63.

DOI:10.1186/1751-0147-53-63

PMID:22118568

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3247080/

Abstract

BACKGROUND

The developmental profile of chicken carbonic anhydrase-III (CA-III) blood levels has not been previously determined or reported. We isolated CA-III from chicken muscle and investigated age-related changes in the levels of CA-III in blood.

METHODS

CA-III was purified from chicken muscle. The levels of CA-III in plasma and erythrocytes from 278 female chickens (aged 1-93 weeks) and 68 male chickens (aged 3-59 weeks) were determined by ELISA.

RESULTS

The mean level of CA-III in female chicken erythrocytes (1 week old) was 4.6 μg/g of Hb, and the CA-III level did not change until 16 weeks of age. The level then increased until 63 weeks of age (11.8 μg/g of Hb), decreased to 4.7 μg/g of Hb at 73 weeks of age, and increased again until 93 weeks of age (8.6 μg/g of Hb). The mean level of CA-III in erythrocytes from male chickens (3 weeks old) was 2.4 μg/g of Hb, and this level remained steady until 59 weeks of age. The mean plasma level of CA-III in 1-week-old female chickens was 60 ng/mL, and this level was increased at 3 weeks of age (141 ng/mL) and then remained steady until 80 weeks of age (122 ng/mL). The mean plasma level of CA-III in 3-week-old male chickens was 58 ng/mL, and this level remained steady until 59 weeks of age.

CONCLUSION

We observed both developmental changes and sex differences in CA-III concentrations in White Leghorn (WL) chicken erythrocytes and plasma. Simple linear regression analysis showed a significant association between the erythrocyte CA-III level and egg-laying rate in WL-chickens 16-63 weeks of age (p < 0.01).

摘要

背景

鸡碳酸酐酶-III（CA-III）血液水平的发育情况尚未被确定或报道过。我们从鸡肌肉中分离出 CA-III，并研究了 CA-III 水平与年龄的关系。

方法

从鸡肌肉中纯化 CA-III。用 ELISA 法测定 278 只母鸡（1-93 周龄）和 68 只公鸡（3-59 周龄）血浆和红细胞中的 CA-III 水平。

结果

母鸡红细胞中 CA-III 的平均水平（1 周龄）为 4.6 μg/g Hb，直到 16 周龄时才发生变化。然后，该水平一直增加到 63 周龄（11.8 μg/g Hb），在 73 周龄时降至 4.7 μg/g Hb，随后又增加到 93 周龄（8.6 μg/g Hb）。公鸡红细胞中 CA-III 的平均水平（3 周龄）为 2.4 μg/g Hb，直到 59 周龄时才保持稳定。1 周龄母鸡的平均血浆 CA-III 水平为 60 ng/mL，在 3 周龄时增加（141 ng/mL），然后在 80 周龄（122 ng/mL）前保持稳定。3 周龄公鸡的平均血浆 CA-III 水平为 58 ng/mL，直到 59 周龄时才保持稳定。