Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu, University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
Acta Vet Scand. 2012 May 29;54(1):34. doi: 10.1186/1751-0147-54-34.
The levels and immunohistochemical localization of muscle carbonic anhydrase III (CA-III) in healthy chickens and in muscular dystrophia affected (DA) chickens show that the muscles of diseased animal undergo a progressive increase of enzyme activity.
An enzyme-linked immunoassay was used to assess the CA-III levels in the muscles and other tissues from eight normal White Leghorn chickens and in two chickens with muscular dystrophy. Immunohistochemical localization of the enzyme in the muscles of these animals was also determined.
The levels of CA-III in the tensor fasciae latae and the superficial pectoral muscles of the DA chickens were higher than the level in normal chickens. The concentrations of CA-III in erythrocytes and plasma from diseased chickens were approximately 15-fold and 1.4-fold higher than in the normal chickens, respectively. In the superficial pectoral and the tensor fasciae latae muscles of diseased chickens, the numbers of strongly stained and weakly stained fibers were greater than that in the normal chickens.
The levels of CA-III in the superficial pectoral muscle, the tensor fasciae latae muscle, plasma and erythrocytes from the chickens with muscular dystrophy were higher than found in normal chickens.
健康鸡和肌肉营养不良症（DA）鸡的肌肉碳酸酐酶 III（CA-III）的水平和免疫组织化学定位表明，患病动物的肌肉酶活性逐渐增加。
使用酶联免疫吸附试验来评估 8 只正常白来航鸡和 2 只肌肉营养不良症鸡的肌肉和其他组织中的 CA-III 水平，并确定这些动物肌肉中酶的免疫组织化学定位。
DA 鸡的阔筋膜张肌和胸浅肌中的 CA-III 水平高于正常鸡。患病鸡的红细胞和血浆中的 CA-III 浓度分别比正常鸡高约 15 倍和 1.4 倍。在患病鸡的胸浅肌和阔筋膜张肌中，强染色和弱染色纤维的数量多于正常鸡。
肌肉营养不良症鸡的胸浅肌、阔筋膜张肌、血浆和红细胞中的 CA-III 水平高于正常鸡。
