Department of Biochemistry, Faculty of Physical Education and Sport in Biala Podlaska, Jozef Pilsudski University of Physical Education in Warsaw, Akademicka 2, 21-500 Biala Podlaska, Poland.
Nutr Res. 2011 Nov;31(11):813-21. doi: 10.1016/j.nutres.2011.09.020.
The purpose of this study was to evaluate the effects of a long-term (4-week) green tea extract (GTE) supplementation in combination with strength training on selected blood markers of oxidative stress and muscular damage after a short-term exercise in previously untrained men. We hypothesized that GTE supplementation would elevate antioxidant potential and attenuate exercise-induced oxidative stress and muscular damage. Thirty-five male students were exposed to 4 weeks of strength training and received (in a randomized, double-blind design) GTE (n = 17; 640 mg polyphenols/d) or placebo (P; n = 18). Before (term I) and after 4 weeks of strength training and supplementation (term II), students performed a short-term muscular endurance test. Blood samples were collected at rest, 5 minutes after the muscular endurance test, and after 24 hours of recovery. Supplementation with GTE enhanced plasma total polyphenols at rest and 5 minutes after the muscular endurance test. Supplementation also contributed to the rise of resting total antioxidant status in plasma. Throughout the experiment (terms I and II), a reduction in plasma lipid hydroxyperoxides was observed 24 hours after the muscular endurance test. Four weeks of strength training resulted in an increase in plasma lipid hydroxyperoxides at rest, but only in the P group. In term I, the muscular endurance test induced an increase in activity of creatine kinase in plasma after 24 hours of recovery in both the P and GTE groups. In term II, plasma creatine kinase activity after 24 hours of recovery was elevated only in the P group. In conclusion, in previously untrained men, dietary supplementation with GTE (in combination with strength training) enhances the antioxidant defense system in plasma at rest and, in turn, may give protection against oxidative damage induced by both short-term muscular endurance test and long-term strength training.
本研究旨在评估长期（4 周）绿茶提取物（GTE）补充与力量训练相结合对未经训练男性进行短期运动后氧化应激和肌肉损伤的某些血液标志物的影响。我们假设 GTE 补充会提高抗氧化能力，并减轻运动引起的氧化应激和肌肉损伤。35 名男性学生接受了 4 周的力量训练，并以随机、双盲的方式接受了 GTE（n=17；640mg 多酚/d）或安慰剂（P；n=18）补充。在力量训练和补充的 4 周前（第 I 期）和之后（第 II 期），学生进行了一项短期肌肉耐力测试。在休息时、肌肉耐力测试后 5 分钟和恢复 24 小时后采集血液样本。GTE 补充使休息时和肌肉耐力测试后 5 分钟时的血浆总多酚含量增加。补充还促进了血浆总抗氧化状态的升高。在整个实验过程中（第 I 期和第 II 期），肌肉耐力测试后 24 小时观察到血浆脂质过氧化物水平降低。4 周的力量训练导致休息时血浆脂质过氧化物水平升高，但仅在 P 组中。在第 I 期，肌肉耐力测试后 24 小时恢复时，P 和 GTE 组的血浆肌酸激酶活性均升高。在第 II 期，仅在 P 组中，恢复 24 小时后血浆肌酸激酶活性升高。总之，在未经训练的男性中，GTE（与力量训练相结合）的饮食补充可增强休息时血浆中的抗氧化防御系统，从而可能对短期肌肉耐力测试和长期力量训练引起的氧化损伤提供保护。
