LIBS, Integrate Biomolecules Laboratory, Federal University of Ceará, Sobral, CE, Brazil.
Arch Oral Biol. 2012 May;57(5):550-5. doi: 10.1016/j.archoralbio.2011.10.016. Epub 2011 Nov 25.
The antibacterial activity of Casbane Diterpene (CD) was evaluated in vitro against Streptococcus oralis, S. mutans, S. salivarius, S. sobrinus, S. mitis and S. sanguinis. The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and antibiofilm action was assayed.
The minimal inhibitory and bactericidal concentrations (MIC and MBC) of oral Streptococcus were evaluated through microdilution tests. To assay antibiofilm activity, biofilms were generated on 96-wells polystyrene plates under the presence of CD and quantified by a crystal violet technique and colonies forming units counting.
The CD isolated from Croton nepetaefolius showed antimicrobial effect on planktonic forms and biofilms of oral pathogens, with MIC values of 62.5 μg/mL against Streptococcus oralis and values between 125 and 500 μg/mL against S. mutans, S. salivarius, S. sobrinus, S. mitis and S. sanguinis. CD showed an inhibitory effect on S. mutans biofilm formation at 250 μg/mL, and a decrease on viable cell of 94.28% compared to the normal biofilm growth.
The compound CD can be considered as a promising molecule for the treatment against oral pathogens responsible for dental biofilm.
评估大麻二萜（CD）对口腔链球菌、变异链球菌、唾液链球菌、远缘链球菌、米勒链球菌和血链球菌的体外抗菌活性。通过药敏试验（MIC 和 MBC）分析浮游细胞的活力，并测定抗生物膜作用。
通过微量稀释试验评估口腔链球菌的最小抑菌和杀菌浓度（MIC 和 MBC）。为了检测抗生物膜活性，在 CD 的存在下，在聚苯乙烯 96 孔板上生成生物膜，并通过结晶紫技术和菌落形成单位计数进行定量。
从尼泊尔叶麻中分离出的 CD 对浮游形式和口腔病原体的生物膜表现出抗菌作用，对口腔链球菌的 MIC 值为 62.5 μg/mL，对变异链球菌、唾液链球菌、远缘链球菌、米勒链球菌和血链球菌的 MIC 值在 125 至 500 μg/mL 之间。CD 在 250 μg/mL 时对变异链球菌生物膜形成具有抑制作用，与正常生物膜生长相比，活菌减少 94.28%。
该化合物 CD 可被视为治疗导致牙生物膜的口腔病原体的有前途的分子。
