Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, North Bengal University, P.O: NBU, Raja Rammohunpur, District, Darjeeling, West Bengal, India.
Botanical Survey of India, CGO Complex, 3rd MSO Building, Salt Lake Sector I, Kolkata, West Bengal, 700 064, India.
BMC Complement Altern Med. 2019 Jul 23;19(1):181. doi: 10.1186/s12906-019-2534-4.
Study of phytochemicals and pharmacological properties of bryophytes has been neglected for a long time because of the three main reasons i.e. (i) difficulty in collection in large amount for analysis; (ii) their availablility only in particular season and (iii) their restricted geographic distribution. So, the aim of this work was to propagate Lunularia cruciata under in vitro condition for comparing its pharmacological properties and phytocmecial constituents with naturally grown counterparts.
Axenic culture of L. cruciata was established by propagating gemmae under in vitro condition. Appropriate culture conditions, media, and the effect of hormones on growth and development were studied. The phytochemical composition was determined by GC-MS analysis and pharmacological activity was evaluated by assessing the antioxidant and anti-diabetic activities. For the antioxidant activity ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)], DPPH (2,2-diphenyl-1-picrylhydrazyl) and metal chelating assays were done and for evaluation of the in vitro anti-diabetic activity α-glucosidase and α-amylase inhibitory activities were done.
Growth of L. cruciata was recorded in half strength MS media. Benzylaminopurine (BAP: 2 mg/L) and 1-Naphtheleneacetic acid (NAA: 0.5 mg/L) were the successful hormonal combination. GC-MS analysis revealed the existence of nine key compounds in both in vitro and naturally grown L. cruciata. Result of antioxidant and anti-diabetic activity showed that in vitro grown L. cruciata has a more or less similar antioxidant and anti-diabetic activities as naturally grown ones. This result confirms the possibility of using in vitro grown plants in place of naturally grown plants for research and clinical purposes.
由于三个主要原因，即 (i) 大量采集进行分析困难；(ii) 仅在特定季节可用；(iii) 地理分布受限，长期以来，人们一直忽视了对苔藓植物的植物化学和药理学特性的研究。因此，本工作的目的是在体外条件下繁殖叉叶曲尾藓，以比较其与天然生长的对应物的药理学特性和植物化学成分。
通过在体外条件下繁殖芽体，建立叉叶曲尾藓的无菌培养。研究了适当的培养条件、培养基和激素对生长和发育的影响。通过 GC-MS 分析确定了植物化学成分，并通过评估抗氧化和抗糖尿病活性来评估其药理活性。为了评估抗氧化活性，进行了 ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)]、DPPH（2,2-二苯基-1-苦基肼）和金属螯合测定，以及体外抗糖尿病活性的α-葡萄糖苷酶和α-淀粉酶抑制活性测定。
在半强度 MS 培养基中记录到叉叶曲尾藓的生长。苄基氨基嘌呤（BAP：2 mg/L）和 1-萘乙酸（NAA：0.5 mg/L）是成功的激素组合。GC-MS 分析表明，在体外和天然生长的叉叶曲尾藓中都存在九种关键化合物。抗氧化和抗糖尿病活性的结果表明，体外生长的叉叶曲尾藓具有或多或少相似的抗氧化和抗糖尿病活性。这一结果证实了在研究和临床目的中使用体外生长的植物代替天然生长的植物的可能性。
