Lartigue-Mattei C, Chabard J L, Bargnoux H, Petit J, Berger J A, Ristori J M, Bussiere J L, Catilina P, Catilina M J
Laboratoire de Chimie Analytique et de Spectrométrie de Masse, Groupe de Recherches en Biodynamique du Médicament, Faculté de Pharmacie, Clermont-Ferrand, France.
J Chromatogr. 1990 Jul 13;529(1):93-101. doi: 10.1016/s0378-4347(00)83810-4.
Plasma and blood xanthine and hypoxanthine levels were assayed using a sensitive and specific method involving gas chromatography-mass spectrometry, associated with an optimized sample preparation procedure. Physiological variation was studied in 224 subjects with no purine metabolism disorders. An age dependency for both compounds was found, comparable with that known for uric acid. The mean plasma levels for the 224 subjects were 0.65 +/- 0.24 microM for xanthine and 1.65 +/- 0.78 microM for hypoxanthine. Corresponding mean blood levels were 0.59 +/- 0.21 microM for xanthine and 1.72 +/- 0.74 microM for hypoxanthine. Plasma and blood levels were significantly different, by ca. 10%. Rapid in vitro release of hypoxanthine from erythrocytes and continuation of intraerythrocytal metabolism lead to overestimation exceeding 10% within half an hour after sample blood collection. Hence samples must be deproteinized promptly. Blood can therefore be conveniently used for oxypurine assay instead of plasma when prompt spinning of samples is difficult to manage, as is usually encountered in clinical practice.
采用一种灵敏且特异的方法，即气相色谱 - 质谱联用并结合优化的样品制备程序，对血浆和血液中的黄嘌呤及次黄嘌呤水平进行了测定。在224名无嘌呤代谢紊乱的受试者中研究了其生理变异情况。发现这两种化合物均存在年龄依赖性，与已知的尿酸情况类似。224名受试者的血浆平均水平为：黄嘌呤0.65±0.24微摩尔/升，次黄嘌呤1.65±0.78微摩尔/升。相应的血液平均水平为：黄嘌呤0.59±0.21微摩尔/升，次黄嘌呤1.72±0.74微摩尔/升。血浆和血液水平存在显著差异，约为10%。红细胞内次黄嘌呤的快速体外释放及红细胞内代谢的持续进行会导致在采集血样后半小时内高估超过10%。因此，样品必须迅速进行脱蛋白处理。当像临床实践中通常遇到的那样难以迅速对样品进行离心时，血液因此可方便地用于氧嘌呤测定，而不用血浆。
