Lartigue-Mattéi C, Chabard J L, Bargnoux H, Petit J, Berger J A, Ristori J M, Bussière J L, Rampon S
Ann Biol Clin (Paris). 1984;42(5):355-61.
The differential assay of xanthine and hypoxanthine in plasma, serum and erythrocytes was performed using a combination of GC and MS with chemical ionisation. The influence of sampling conditions was studied, in particular the latency period between the collection of the blood and the separation of the plasma and the cells, the nature of the anticoagulant used and the method of storage of the samples. Firstly, this study confirms that EDTA is the most appropriate anticoagulant. It also showed that an immediate deproteinisation is necessary after separation of the plasma and the cells, in order to prevent any "in vitro" modification of the oxypurines, in particular erythrocyte hypoxanthine.
采用气相色谱和化学电离质谱联用技术对血浆、血清和红细胞中的黄嘌呤和次黄嘌呤进行差异分析。研究了采样条件的影响，特别是采血与血浆和细胞分离之间的延迟期、所用抗凝剂的性质以及样品的储存方法。首先，本研究证实乙二胺四乙酸（EDTA）是最合适的抗凝剂。研究还表明，血浆和细胞分离后必须立即进行脱蛋白处理，以防止氧嘌呤特别是红细胞次黄嘌呤发生任何“体外”修饰。
