非理想条件下商业运输介质对生物病原体的适用性
Suitability of Commercial Transport Media for Biological Pathogens under Nonideal Conditions.
作者信息
Hubbard Kyle, Pellar Gregory, Emanuel Peter
机构信息
Excet, Inc., 8001 Braddock Rd Suite 303, Springfield, VA 22151, USA.
出版信息
Int J Microbiol. 2011;2011:463096. doi: 10.1155/2011/463096. Epub 2011 Oct 30.
There is extensive data to support the use of commercial transport media as a stabilizer for known clinical samples; however, there is little information to support their use outside of controlled conditions specified by the manufacturer. Furthermore, there is no data to determine the suitability of said media for biological pathogens, specifically those of interest to the US military. This study evaluates commercial off-the-shelf (COTS) transport media based on sample recovery, viability, and quality of nucleic acids and peptides for nonpathogenic strains of Bacillus anthracis, Yersinia pestis, and Venezuelan equine encephalitis virus, in addition to ricin toxin. Samples were stored in COTS, PBST, or no media at various temperatures over an extended test period. The results demonstrate that COTS media, although sufficient for the preservation of nucleic acid and proteinaceous material, are not capable of maintaining an accurate representation of biothreat agents at the time of collection.
有大量数据支持将商用运输介质用作已知临床样本的稳定剂；然而，几乎没有信息支持在制造商规定的受控条件之外使用它们。此外，没有数据来确定所述介质对生物病原体的适用性，特别是对美军感兴趣的那些病原体。本研究评估了商用现货（COTS）运输介质，这些介质用于炭疽芽孢杆菌、鼠疫耶尔森菌和委内瑞拉马脑炎病毒的非致病菌株以及蓖麻毒素的样本回收、活力以及核酸和肽的质量。在延长的测试期内，样本分别储存在商用现货介质、磷酸盐缓冲盐水（PBST）或无介质中，并置于不同温度下。结果表明，商用现货介质虽然足以保存核酸和蛋白质材料，但在采集时无法准确呈现生物威胁剂的情况。
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