Choi Hyun, Choi Insung S, Lee Gil Sun, Ahn Dong June
Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea.
J Nanosci Nanotechnol. 2011 Jul;11(7):6203-7. doi: 10.1166/jnn.2011.4403.
Polydiacetylenes provide a good biological/chemical label-free detection system owing to their blue to red color change and fluorescence change from non-fluorescence to red fluorescence. One of the important factors to consider before applying polydiacetylenes to various sensors is to enhance the sensitivity against specific molecules. This paper reports two methods for stacking polydiacetylene vesicles for fluorescence signal enhancement of polydiacetylene on solid substrates. This focuses on how to achieve a highly sensitive sensor chip by introducing two strategies to immobilize the vesicles effectively on substrates. One method is layer-by-layer deposition through reactions of vesicles and avidins alternately on an avidin treated substrate. The other is to prepare a clustered vesicles solution by mixing the appropriate amount of a biotinylated vesicle solution and avidin solution, and then immobilize the clustered vesicles on the substrates. The former allows easy control of the layer thickness, whereas the latter can shorten the process time. These strategies can be applied to a range of biological/chemical sensors for fluorescence signal enhancement.
聚二乙炔由于其从蓝色到红色的颜色变化以及从非荧光到红色荧光的荧光变化，提供了一种良好的无生物/化学标记检测系统。在将聚二乙炔应用于各种传感器之前，需要考虑的一个重要因素是提高对特定分子的灵敏度。本文报道了两种堆叠聚二乙炔囊泡的方法，以增强聚二乙炔在固体基质上的荧光信号。这主要关注如何通过引入两种策略将囊泡有效地固定在基质上来实现高灵敏度的传感器芯片。一种方法是通过囊泡与抗生物素蛋白在抗生物素蛋白处理过的基质上交替反应进行逐层沉积。另一种方法是通过混合适量的生物素化囊泡溶液和抗生物素蛋白溶液制备聚集囊泡溶液，然后将聚集囊泡固定在基质上。前者便于控制层厚度，而后者可以缩短工艺时间。这些策略可应用于一系列用于增强荧光信号的生物/化学传感器。
