Reppy Mary A
Analytical Biological Services Inc., 701-4 Cornell Business Park, Wilmington, DE 19801, USA.
J Fluoresc. 2008 Mar;18(2):461-71. doi: 10.1007/s10895-007-0287-9. Epub 2007 Dec 20.
Enhancement of the environmentally responsive fluorescent properties of polydiacetylene (PDA) by combination with lipophilic fluorophores was demonstrated and properties of the PDA/fluorophore systems were explored. Liposomes containing PDA and fluorophores exhibited enhanced Stokes shift and increase in emission as a result of energy transfer from PDA to fluorophore. The effects of fluorophore variation, degree of PDA polymerization and diyne placement in the diacetylene lipid tails on the emission enhancement were studied. It was determined that signal generation was optimized at a relatively low extent of PDA polymerization with the optimal degree of polymerization dependent on the other parameters. Energy transfer was used as a tool to detect fluorophore exchange between polymerized and unpolymerized liposomes and to study the effects of fluorophore structure on exchange from unpolymerized to PDA liposomes. Fluorophores that locate at the aqueous interface with alkyl anchors were slow to transfer while fluorophores that partition into the alkyl regions of the liposomes transfer quickly.
通过与亲脂性荧光团结合来增强聚二乙炔（PDA）的环境响应荧光特性得到了证实，并对PDA/荧光团体系的性质进行了探索。含有PDA和荧光团的脂质体由于能量从PDA转移到荧光团，表现出增强的斯托克斯位移和发射增加。研究了荧光团变化、PDA聚合度以及二乙炔脂质尾部中二炔位置对发射增强的影响。确定在相对较低的PDA聚合程度下信号产生达到最佳，最佳聚合度取决于其他参数。能量转移被用作一种工具来检测聚合和未聚合脂质体之间的荧光团交换，并研究荧光团结构对从未聚合脂质体到PDA脂质体交换的影响。位于具有烷基锚定基团的水界面处的荧光团转移缓慢，而分配到脂质体烷基区域的荧光团转移迅速。
