基于吲哚菁绿的光动力疗法联合 785nm 发光二极管治疗口腔鳞状癌细胞。
Indocyanine green-based photodynamic therapy with 785nm light emitting diode for oral squamous cancer cells.
机构信息
Department of Medical Laser, Graduate school, Dankook University, Cheonan 330-714, South Korea.
出版信息
Photodiagnosis Photodyn Ther. 2011 Dec;8(4):337-42. doi: 10.1016/j.pdpdt.2011.06.002. Epub 2011 Jul 12.
BACKGROUND
The efficiency of photodynamic therapy (PDT) used in combination with Indocyanine green (ICG) and the light emitting diode (LED) on oral cancer was evaluated. The safety risk of ICG is known to be very low and ICG has a strong peak in the vicinity of 700-800nm range which is thought to be a good candidate as a photosensitizer for PDT due to the deep penetration depth into the oral cancer tissue.
METHODS
The radiation intensity of homemade LED array was 50mW/cm(2) at 0.5A. To evaluate the maximum efficiency of ICG-PDT on oral cancer, different wavelengths, ICG concentrations, irradiation interval times after administering ICG, and the time durations after PDT were tested. The cytotoxicity was determined by MTT assay, and apoptosis and necrosis were also observed by double staining with SYTO 16 green and PI.
RESULTS
The IC(50) value was 10μM when 785nm was irradiated, while it was very low in comparison with 630nm and 895nm. The values were not very different with varying interval time. The percentage of apoptotic cells increased gradually to 84% at 6h after 20μM ICG-PDT and the percentage of necrotic cells dramatically increased to 65% at 3h after 200μM ICG-PDT.
CONCLUSION
Using ICG-PDT with 785nm LED light, the LED is regarded as a satisfying light source since cancer treatments in the oral region do not require focusing and increased depth of penetration due to longer wavelength enhances treatment effectiveness.
背景
评估了光动力疗法（PDT）与吲哚菁绿（ICG）和发光二极管（LED）联合用于口腔癌的疗效。已知 ICG 的安全风险非常低，并且 ICG 在 700-800nm 范围内具有很强的峰值，由于其对口腔癌组织有较深的穿透深度，因此被认为是 PDT 的理想光敏剂。
方法
自制 LED 阵列的辐射强度在 0.5A 时为 50mW/cm²。为了评估 ICG-PDT 在口腔癌中的最大效率，测试了不同波长、ICG 浓度、ICG 给药后照射间隔时间以及 PDT 后时间持续时间。通过 MTT 测定法测定细胞毒性，并用 SYTO 16 绿色和 PI 双重染色观察细胞凋亡和坏死。
结果
当用 785nm 照射时，IC₅₀值为 10μM，而与 630nm 和 895nm 相比非常低。间隔时间不同时，数值没有太大差异。用 20μM ICG-PDT 治疗 6 小时后，凋亡细胞的百分比逐渐增加到 84%，用 200μM ICG-PDT 治疗 3 小时后，坏死细胞的百分比急剧增加到 65%。
结论
使用 785nm LED 光的 ICG-PDT，由于长波长会增强治疗效果，因此 LED 被认为是一种令人满意的光源，因为口腔区域的癌症治疗不需要聚焦和增加穿透深度。
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