Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Chelmska Street 30/34, Warsaw, Poland.
Antonie Van Leeuwenhoek. 2012 Mar;101(3):671-5. doi: 10.1007/s10482-011-9681-z. Epub 2011 Nov 29.
This study aimed to assess the efficiency of the Cepheid Xpert vanA/vanB test for detecting vancomycin-resistant enterococci (VRE) colonization during a VanA Enterococcus faecium outbreak and to compare the Cepheid Xpert vanA/vanB (Cepheid, Sunnyvale, USA) test to a culture method with chromogenic medium chromID VRE agar (bioMérieux). The Cepheid Xpert vanA/vanB assay showed sensitivity 61.5%, specificity 79.2%, positive predictive value 61.5% and negative predictive value 79.2%. The results obtained in this study demonstrate that a positive result in the Cepheid Xpert vanA/vanB test for vanA enables the rapid (less than 1 h) presumptive, prior to culture, recognition of patients colonized with VRE. However, the Cepheid Xpert vanA/vanB assay cannot be the only test used to screen patients during an ongoing VRE outbreak, because additional culturing of all samples negative for both vanA and vanB or positive for vanB should be performed in order to confirm the carrier status of the patient.
本研究旨在评估 Cepheid Xpert vanA/vanB 检测在 VanA 肠球菌爆发期间检测耐万古霉素肠球菌（VRE）定植的效率，并比较 Cepheid Xpert vanA/vanB（Cepheid，美国桑尼维尔）检测与显色培养基 chromID VRE 琼脂（bioMérieux）的培养方法。Cepheid Xpert vanA/vanB 检测的灵敏度为 61.5%，特异性为 79.2%，阳性预测值为 61.5%，阴性预测值为 79.2%。本研究的结果表明，Cepheid Xpert vanA/vanB 检测对 vanA 的阳性结果可在培养前快速（小于 1 小时）推定识别 VRE 定植的患者。然而，Cepheid Xpert vanA/vanB 检测不能作为在正在进行的 VRE 爆发期间筛选患者的唯一检测方法，因为应对所有 vanA 和 vanB 均为阴性或 vanB 为阳性的样本进行进一步培养，以确认患者的携带状态。
