Drews Steven J, Johnson Grant, Gharabaghi Farhad, Roscoe Margaret, Matlow Anne, Tellier Raymond, Richardson Susan E
Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, 555 University Ave., Room 3654, Toronto, Ontario M5G 1X8, Canada.
J Clin Microbiol. 2006 Apr;44(4):1578-80. doi: 10.1128/JCM.44.4.1578-1580.2006.
We describe a 24-h protocol for the identification of patients who are positive for vancomycin-resistant Enterococcus faecium (VRE), using stool and rectal swab samples and VRE screening broth, automated DNA extraction, and real-time PCR for vanA and vanB genes. Compared to conventional screening methods, this protocol had a high sensitivity and specificity and a negative predictive value.
我们描述了一种24小时方案,用于鉴定耐万古霉素屎肠球菌(VRE)阳性患者,该方案使用粪便和直肠拭子样本、VRE筛选肉汤、自动化DNA提取以及针对vanA和vanB基因的实时PCR。与传统筛选方法相比,该方案具有高灵敏度、高特异性和阴性预测值。