一种用于鉴定耐万古霉素屎肠球菌的24小时筛查方案。
A 24-hour screening protocol for identification of vancomycin-resistant Enterococcus faecium.
作者信息
Drews Steven J, Johnson Grant, Gharabaghi Farhad, Roscoe Margaret, Matlow Anne, Tellier Raymond, Richardson Susan E
机构信息
Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, 555 University Ave., Room 3654, Toronto, Ontario M5G 1X8, Canada.
出版信息
J Clin Microbiol. 2006 Apr;44(4):1578-80. doi: 10.1128/JCM.44.4.1578-1580.2006.
Abstract
We describe a 24-h protocol for the identification of patients who are positive for vancomycin-resistant Enterococcus faecium (VRE), using stool and rectal swab samples and VRE screening broth, automated DNA extraction, and real-time PCR for vanA and vanB genes. Compared to conventional screening methods, this protocol had a high sensitivity and specificity and a negative predictive value.
摘要
我们描述了一种24小时方案，用于鉴定耐万古霉素屎肠球菌（VRE）阳性患者，该方案使用粪便和直肠拭子样本、VRE筛选肉汤、自动化DNA提取以及针对vanA和vanB基因的实时PCR。与传统筛选方法相比，该方案具有高灵敏度、高特异性和阴性预测值。
相似文献
1
A 24-hour screening protocol for identification of vancomycin-resistant Enterococcus faecium.一种用于鉴定耐万古霉素屎肠球菌的24小时筛查方案。
J Clin Microbiol. 2006 Apr;44(4):1578-80. doi: 10.1128/JCM.44.4.1578-1580.2006.
2
Efficiency of the Cepheid Xpert vanA/vanB assay for screening of colonization with vancomycin-resistant enterococci during hospital outbreak.Cepheid Xpert vanA/vanB assay 在医院爆发期间筛查耐万古霉素肠球菌定植的效率。
Antonie Van Leeuwenhoek. 2012 Mar;101(3):671-5. doi: 10.1007/s10482-011-9681-z. Epub 2011 Nov 29.
3
Screening for vancomycin-resistant enterococci: results of a survey in Stockholm.万古霉素耐药肠球菌筛查：斯德哥尔摩调查结果。
APMIS. 2010 May;118(5):413-7. doi: 10.1111/j.1600-0463.2010.02605.x.
4
5
Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci: interference by vanB-containing anaerobic bacilli.三种用于鉴定粪便中vanB基因携带情况的PCR引物组的比较及其与耐万古霉素肠球菌携带情况的相关性：含vanB厌氧杆菌的干扰
Antimicrob Agents Chemother. 2005 Jan;49(1):77-81. doi: 10.1128/AAC.49.1.77-81.2005.
6
Experience with the Roche LightCycler VRE detection kit during a large outbreak of vanB2/B3 vancomycin-resistant Enterococcus faecium.罗氏LightCycler VRE检测试剂盒在vanB2/B3型耐万古霉素屎肠球菌大规模暴发期间的应用经验。
J Antimicrob Chemother. 2007 Jul;60(1):182-3. doi: 10.1093/jac/dkm140. Epub 2007 May 4.
7
Structure and transfer of the vanA cluster in vanA-positive, vancomycin-susceptible Enterococcus faecium, and its revertant mutant.vanA 阳性、对万古霉素敏感的屎肠球菌中 vanA 基因簇的结构与转移及其回复突变体
Diagn Microbiol Infect Dis. 2014 Oct;80(2):148-50. doi: 10.1016/j.diagmicrobio.2014.06.012. Epub 2014 Jun 21.
8
Molecular epidemiology of vancomycin-resistant Enterococcus faecium in Argentina.阿根廷耐万古霉素屎肠球菌的分子流行病学
Int J Infect Dis. 2007 Jan;11(1):69-75. doi: 10.1016/j.ijid.2006.02.003. Epub 2006 Jun 21.
9
Evaluation of GeneOhm VanR and Xpert vanA/vanB molecular assays for the rapid detection of vancomycin-resistant enterococci.评估 GeneOhm VanR 和 Xpert vanA/vanB 分子检测法快速检测万古霉素耐药肠球菌。
Eur J Clin Microbiol Infect Dis. 2012 Mar;31(3):273-6. doi: 10.1007/s10096-011-1306-y. Epub 2011 Jun 12.
10
High frequency of vancomycin-resistant Enterococcus faecium isolates with VanB phenotype and vanA genotype in Korean hospitals.韩国医院中具有VanB表型和vanA基因型的耐万古霉素屎肠球菌分离株的高频率。
Diagn Microbiol Infect Dis. 2006 Dec;56(4):401-6. doi: 10.1016/j.diagmicrobio.2006.06.007. Epub 2006 Jul 28.
引用本文的文献
1
Low vancomycin MICs and fecal densities reduce the sensitivity of screening methods for vancomycin resistance in Enterococci.低万古霉素最低抑菌浓度（MIC）和粪便密度会降低肠球菌中万古霉素耐药性筛查方法的敏感性。
J Clin Microbiol. 2014 Aug;52(8):2829-33. doi: 10.1128/JCM.00021-14. Epub 2014 May 28.
2
Evaluation of vancomycin resistance 3 multiplexed PCR assay for detection of vancomycin-resistant enterococci from rectal swabs.用于从直肠拭子中检测耐万古霉素肠球菌的万古霉素耐药性3重多重聚合酶链反应检测法的评估
Ann Lab Med. 2013 Sep;33(5):326-30. doi: 10.3343/alm.2013.33.5.326. Epub 2013 Aug 8.
3
An outbreak of vancomycin-resistant Enterococcus faecium in an acute care pediatric hospital: Lessons from environmental screening and a case-control study.万古霉素耐药粪肠球菌在一家急性护理儿科医院的爆发：环境筛查和病例对照研究的经验教训。
Can J Infect Dis Med Microbiol. 2008 May;19(3):233-6. doi: 10.1155/2008/727062.
4
Peptide nucleic acid fluorescent in situ hybridization for hospital-acquired enterococcal bacteremia: delivering earlier effective antimicrobial therapy.用于医院获得性肠球菌菌血症的肽核酸荧光原位杂交：实现更早的有效抗菌治疗
Antimicrob Agents Chemother. 2008 Oct;52(10):3558-63. doi: 10.1128/AAC.00283-08. Epub 2008 Jul 28.
5
Current trends in rapid diagnostics for methicillin-resistant Staphylococcus aureus and glycopeptide-resistant enterococcus species.耐甲氧西林金黄色葡萄球菌和耐糖肽肠球菌快速诊断的当前趋势。
J Clin Microbiol. 2008 May;46(5):1577-87. doi: 10.1128/JCM.00326-08. Epub 2008 Mar 5.
6
Utility of direct, real-time PCR in the management of a nosocomial outbreak of vancomycin-resistant Enterococcus faecium (vanB genotype).直接实时聚合酶链反应在耐万古霉素屎肠球菌（vanB基因型）医院感染暴发管理中的应用
Eur J Clin Microbiol Infect Dis. 2008 Apr;27(4):321-2. doi: 10.1007/s10096-007-0432-z. Epub 2007 Dec 18.
7
Comparison of the BD GeneOhm VanR assay to culture for identification of vancomycin-resistant enterococci in rectal and stool specimens.在直肠和粪便标本中，将BD GeneOhm VanR检测法与培养法用于鉴定耐万古霉素肠球菌的比较。
J Clin Microbiol. 2007 Oct;45(10):3360-5. doi: 10.1128/JCM.01458-07. Epub 2007 Aug 17.
本文引用的文献
1
Implications of treatment-induced mucosal barrier injury.治疗引起的黏膜屏障损伤的影响。
Curr Opin Oncol. 2005 Nov;17(6):605-10.
2
Management of bleeding in the terminally ill patient.晚期患者出血的管理。
Hematology. 2005 Jun;10(3):167-75. doi: 10.1080/10245330500093237.
3
Comparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin-resistant enterococci from perianal swabs.罗氏LightCycler vanA/vanB检测法与培养法用于检测肛周拭子中耐万古霉素肠球菌的比较。
J Clin Microbiol. 2004 Jun;42(6):2636-43. doi: 10.1128/JCM.42.6.2636-2643.2004.
4
Rapid detection of vanA and vanB genes directly from clinical specimens and enrichment broths by real-time multiplex PCR assay.通过实时多重聚合酶链反应检测法直接从临床标本和增菌肉汤中快速检测vanA和vanB基因。
J Clin Microbiol. 2003 Jun;41(6):2483-6. doi: 10.1128/JCM.41.6.2483-2486.2003.
5
Clinical prevalence, antimicrobial susceptibility, and geographic resistance patterns of enterococci: results from the SENTRY Antimicrobial Surveillance Program, 1997-1999.肠球菌的临床患病率、抗菌药物敏感性及地域耐药模式：1997 - 1999年哨兵抗菌监测计划的结果
Clin Infect Dis. 2001 May 15;32 Suppl 2:S133-45. doi: 10.1086/320185.
6
Vancomycin-resistant enterococci.耐万古霉素肠球菌
Semin Respir Infect. 2000 Dec;15(4):314-26. doi: 10.1053/srin.2000.20945.
7
Preventing the spread of vancomycin-resistant enterococci (VRE) in Canada.在加拿大预防耐万古霉素肠球菌（VRE）的传播。
Can Commun Dis Rep. 1997 Dec;23 Suppl 8:i-iv, 1-16, i-iv, 1-19.
8
Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.用于人类布鲁氏菌病诊断的外周血PCR检测中优化DNA扩增的策略。
J Clin Microbiol. 1998 Sep;36(9):2443-6. doi: 10.1128/JCM.36.9.2443-2446.1998.
9
Epidemiologic analysis and genotypic characterization of a nosocomial outbreak of vancomycin-resistant enterococci.耐万古霉素肠球菌医院感染暴发的流行病学分析及基因分型特征
J Clin Microbiol. 1993 May;31(5):1280-5. doi: 10.1128/jcm.31.5.1280-1285.1993.
10
Emergence of vancomycin-resistant enterococci in New York City.纽约市耐万古霉素肠球菌的出现。
Lancet. 1993 Jul 10;342(8863):76-9. doi: 10.1016/0140-6736(93)91285-t.
Zotero 插件