Department of Otorhinolaryngology-Head and Neck Surgery, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Republic of Korea.
Ann Surg Oncol. 2012 May;19(5):1595-601. doi: 10.1245/s10434-011-2154-9. Epub 2011 Nov 29.
To investigate the change of vasculature in the sentinel lymph node (SLN) of patients with oral squamous cell carcinoma.
Immunohistochemical staining of SLNs in 58 patients was performed with two monoclonal antibodies (MAb): anti-D2-40 MAb for lymphatic endothelial cells, and anti-MECA-79 MAb for high endothelial venules (HEV). Twelve metastatically involved (m(+)) SLNs, 120 uninvolved (m(-)) SLNs, and 35 non-SLNs (control) were available for analyses. Vessel densities were measured by computer-assisted analyses in the entire region of SLN. Correlations were assessed between vessel density and clinicopathologic variables, including vascular endothelial growth factor C of primary tumor.
Lymphatic vessel density (LVD) in SLNs was higher than that in control LNs [2361.8 μm(2)/high-power field (HPF) (624.3-4758.5) vs. 1621.9 μm(2)/HPF (465.3-3453.5), P = 0.005]. LVD of m(-) SLNs [2662.4 μm(2)/HPF (624.3-4758.5)] and m(±) SLNs [4946.6 μm(2)/HPF (2009.3-8698.8)] were both statistically significantly higher compared to control. HEV densities in m(-) SLNs [14029.7 μm(2)/HPF (10465.7-17927.1)] as well as m(±) SLNs [18258.5 μm(2)/HPF (8408.9-27706.0)] were also significantly higher than those in control [10350.5 μm(2)/HPF (7807.8-12541.1)]. By multivariate analysis, the degree of vascular endothelial growth factor C expression of primary tumor showed significant correlation with LVD of SLNs (odds ratio 9.46, 95% confidence interval 1.73-51.5, P = 0.009), which was not the case in HEV.
Lymphatic vessels and HEVs were increased in SLNs, regardless of metastatic status of SLNs. Vascular endothelial growth factor C expression of primary tumor may contribute to the premetastatic change within SLNs of oral squamous cell carcinoma.
研究口腔鳞状细胞癌前哨淋巴结（SLN）中脉管系统的变化。
对 58 例患者的 SLN 进行了两种单克隆抗体（MAb）的免疫组织化学染色：抗 D2-40 MAb 用于淋巴管内皮细胞，抗 MECA-79 MAb 用于高内皮静脉（HEV）。12 个转移性受累（m(+））SLN、120 个未受累（m(-））SLN 和 35 个非 SLN（对照）可用于分析。通过计算机辅助分析测量整个 SLN 区域的血管密度。评估血管密度与临床病理变量之间的相关性，包括原发性肿瘤的血管内皮生长因子 C。
SLN 中的淋巴管密度（LVD）高于对照淋巴结[2361.8μm(2)/高倍视野（HPF）（624.3-4758.5）与 1621.9μm(2)/HPF（465.3-3453.5），P=0.005]。m(-)SLN 的 LVD[2662.4μm(2)/HPF（624.3-4758.5）]和 m(±)SLN 的 LVD[4946.6μm(2)/HPF（2009.3-8698.8）]均明显高于对照。m(-)SLN[14029.7μm(2)/HPF（10465.7-17927.1）]和 m(±)SLN[18258.5μm(2)/HPF（8408.9-27706.0）]中的 HEV 密度也明显高于对照[10350.5μm(2)/HPF（7807.8-12541.1）]。多变量分析显示，原发性肿瘤血管内皮生长因子 C 的表达程度与 SLN 的 LVD 呈显著相关（比值比 9.46，95%置信区间 1.73-51.5，P=0.009），而 HEV 则不然。
无论 SLN 的转移状态如何，SLN 中的淋巴管和 HEV 均增加。原发性肿瘤血管内皮生长因子 C 的表达可能有助于口腔鳞状细胞癌 SLN 内的前转移变化。
