Ayromlou Hormoz, Hajipour Babak, Hossenian Mohammad Mehdi, Khodadadi Ali, Vatankhah Amir Mansour
Neurology Research Center, Islamic Azad University of Tabriz, Tabriz City, Iran.
J Pak Med Assoc. 2011 Nov;61(11):1096-9.
To study the potential effect of Methotrexate (MTX) on lipid peroxidation and activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD) two important endogenous anti-oxidant enzymes.
Twenty healthy male Newzland rabbits weighting 1500-1800 g were divided into two groups. One group was considered as the MTX groups and the other was considered as control group. Rabbits had free access to food and tap water. Rabbits in MTX group received a single dose of MTX, 20 mg/kg intraperitoneally, a similar volume of saline was administered to control group. After 6 days rabbits were uteinized and spinal tissue excised for biochemical studies.
GPX and SOD activities in spinal tissue in rabbits of MTX group significantly reduced after MTX administration compared to control group(P = 0.002 vs 0.18). An increase in the tissue MDA level was seen in the MTX group, suggesting increased lipid peroxidation. Levels of MDA were significantly higher in MTX group compared to control group (P = 0.002).
Our data suggests that MTX treatment induces oxidative tissue damage on the spinal tissue, as assessed by increased lipid peroxidation and decreased GPX and SOD levels, so decreasing oxidative stress by anti-oxidant agents may play a key role in attenuating spinal cord injury.
研究甲氨蝶呤（MTX）对脂质过氧化以及两种重要的内源性抗氧化酶——谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）活性的潜在影响。
将20只体重为1500 - 1800克的健康雄性新西兰兔分为两组。一组为MTX组，另一组为对照组。兔子可自由进食和饮用自来水。MTX组兔子腹腔注射单剂量MTX，20毫克/千克，对照组注射相同体积的生理盐水。6天后处死兔子，切除脊髓组织进行生化研究。
与对照组相比，MTX组兔子脊髓组织中的GPX和SOD活性在给予MTX后显著降低（P = 0.002对0.18）。MTX组组织MDA水平升高，提示脂质过氧化增加。MTX组MDA水平显著高于对照组（P = 0.002）。
我们的数据表明，MTX治疗可诱导脊髓组织发生氧化损伤，这可通过脂质过氧化增加以及GPX和SOD水平降低来评估，因此使用抗氧化剂降低氧化应激可能在减轻脊髓损伤中起关键作用。
