肺癌患者的脂质过氧化、DNA损伤与辅酶Q10——风险评估指标？
Lipid peroxidation, DNA damage and coenzyme Q10 in lung cancer patients--markers for risk assessment?
作者信息
Cobanoglu Ufuk, Demir Halit, Cebi Aysegul, Sayir Fuat, Alp Hamit Hakan, Akan Zafer, Gur Tugba, Bakan Ebubekir
机构信息
Department of Thoracic Surgery, Divison of Biochemistry, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
出版信息
Asian Pac J Cancer Prev. 2011;12(6):1399-403.
OBJECTIVES
Early diagnosis and prevention is very important for lung cancer patients. Previous studies have emphasized that the level of coenzyme Q10 (CoQ10), present primarily in mitochondria, decreases with age and is low in patients with chronic diseases. Our goal was to find out if there is any relationship between lung cancer and CoQ10 and lipid peroxidation levels.
DESIGN AND METHODS
Blood samples from lung cancer patients were collected. Total and oxide CoQ10 levels, 8-OHdG (product of DNA damage), and malondialdehyde (MDA) levels (lipid peroxidation) were analyzed with high performance liquid chromatography (HPLC).
RESULTS
The MDA level (P<0.001) and DNA damage rate (8-OHdG) (P<0.001) was higher in cancer patients than in the control group; in contrast, theCoQ10 enzyme level was significantly lower (P<0.001).
CONCLUSION
The results suggest that the aforementioned parameters can be useful for lung cancer risk assessment.
目的
早期诊断和预防对肺癌患者非常重要。先前的研究强调，主要存在于线粒体中的辅酶Q10（CoQ10）水平会随着年龄增长而降低，并且在慢性病患者中含量较低。我们的目标是找出肺癌与CoQ10以及脂质过氧化水平之间是否存在任何关联。
设计与方法
采集肺癌患者的血样。采用高效液相色谱法（HPLC）分析总CoQ10和氧化型CoQ10水平、8-羟基脱氧鸟苷（DNA损伤产物）以及丙二醛（MDA）水平（脂质过氧化）。
结果
癌症患者的MDA水平（P<0.001）和DNA损伤率（8-羟基脱氧鸟苷）（P<0.001）高于对照组；相比之下，CoQ10酶水平显著更低（P<0.001）。
结论
结果表明上述参数可用于肺癌风险评估。
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