G Sankara Narayanan, Sssn Rajasekhar, Chaturvedula Latha, Adole Prashant
Anatomy, Saveetha Medical College and Hospital, Chennai, IND.
Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, IND.
Cureus. 2024 Jan 19;16(1):e52600. doi: 10.7759/cureus.52600. eCollection 2024 Jan.
Purpose The objective of this research project was to estimate DNA damage in patients diagnosed with cervical cancer using the comet assay, establish a correlation between this quantification and the oxidative stress marker malondialdehyde (MDA; plasma MDA), and compare the resulting parameters between the cases and age-matched controls. Materials and methods This study included 49 cervical cancer cases and 49 age-matched controls to measure DNA damage parameters such as comet length, head diameter, percentage of DNA in the comet head, tail length, percentage of DNA in the comet tail, and oxidative stress marker (plasma MDA) using the thiobarbituric acid reactive substance (TBARS) enzyme-linked immunosorbent assay (ELISA) method. Results Comet metrics suggesting DNA damage, such as comet length, tail length, and percentage of DNA in the comet tail, were considerably higher in cervical cancer cases than in age-matched controls. The proportion of DNA in the comet head, representing undamaged/mild DNA damage, was significantly higher in age-matched controls than in cervical cancer patients. Plasma MDA and comet tail length were shown to have a positive correlation. Compared to the age-matched controls, those between the ages of 30 and 39, with a parity of two to four, who had a history of early age at first pregnancy and a positive family history of cervical cancer, had the highest level of DNA damage. Conclusion The elevated levels of comet parameters and their positive correlation with plasma MDA suggest that individuals diagnosed with cervical cancer have a higher degree of DNA damage compared to the control group. In conjunction with established methods like the PAP smear, this predictive test comprising comet assay and estimation of plasma MDA may be utilized to identify and assess the risk of cervical cancer in individuals aged 30-39 years, with a parity between two and four pregnancies and a prior history of early age at first pregnancy, accompanied by a positive family history of the disease.
目的 本研究项目的目的是使用彗星试验评估被诊断为宫颈癌的患者的DNA损伤，建立这种量化与氧化应激标志物丙二醛（MDA；血浆MDA）之间的相关性，并比较病例组和年龄匹配对照组之间的所得参数。
材料与方法 本研究纳入了49例宫颈癌病例和49例年龄匹配的对照，以使用硫代巴比妥酸反应性物质（TBARS）酶联免疫吸附测定（ELISA）方法测量DNA损伤参数，如彗星长度、头部直径、彗星头部DNA百分比、尾巴长度、彗星尾巴DNA百分比以及氧化应激标志物（血浆MDA）。
结果 提示DNA损伤的彗星指标，如彗星长度、尾巴长度和彗星尾巴DNA百分比，在宫颈癌病例中显著高于年龄匹配的对照组。代表未受损/轻度DNA损伤的彗星头部DNA比例，在年龄匹配的对照组中显著高于宫颈癌患者。血浆MDA与彗星尾巴长度呈正相关。与年龄匹配的对照组相比，年龄在30至39岁之间、产次为2至4次、有早育史且有宫颈癌家族史阳性的人群，DNA损伤水平最高。
结论 彗星参数水平升高及其与血浆MDA的正相关表明，与对照组相比，被诊断为宫颈癌的个体具有更高程度的DNA损伤。结合巴氏涂片等既定方法，这种包括彗星试验和血浆MDA估计的预测性检测可用于识别和评估年龄在30 - 39岁、产次在2至4次之间且有早育史并伴有该病家族史阳性的个体患宫颈癌的风险。
