Kolusari A, Cebi A, Akgun E, Alp H H, Bakan E
Clin Exp Obstet Gynecol. 2017;44(3):398-402.
The pathological mechanisms of gestational trophoblastic disease have not yet been clearly determined. It is thought that oxidative damage contributes to the process. The aim of this study was to determine the levels of coenzyme Q10 (CoQ 10), DNA damage, and lipid peroxidation in patients with hydatidiform mole.
The authors studied the levels of CoQ10, 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA) by high-performance liquid chromatography (HPLC), and the activity of glutathione peroxidase (GPX) by spectrophotometric method in blood obtained from patients with a complete hydatidiform mole (n=29), healthy pregnant women (n=29), and healthy non-pregnant women (n=29).
The 8-OHdG/dG ratio (2.8148 ± 0.81592) and MDA (10.8341 ± 4.64875 μmol) were significantly higher in patients with complete hydatidiform mole, while the ubiquinol-10/ubiquinone-10 ratio (0.2107 ± 0.15675) and GPX activity (43.4606 ± 18.31694 mU/mI) were lower (p < 0.001).
The authors suggest that both mitochondrial oxidative and oxidative DNA damage play important roles in the pathogenesis of complete hydatidiform mole. Therefore supplementation of CoQ10 prevents recurrent gestational trophoblastic disease.
妊娠滋养细胞疾病的病理机制尚未明确。有观点认为氧化损伤参与了这一过程。本研究旨在测定葡萄胎患者体内辅酶Q10（CoQ10）水平、DNA损伤及脂质过氧化情况。
作者采用高效液相色谱法（HPLC）测定了完全性葡萄胎患者（n = 29）、健康孕妇（n = 29）及健康非孕妇（n = 29）血液中CoQ10、8 - 羟基脱氧鸟苷（8 - OHdG）、丙二醛（MDA）的水平，并通过分光光度法测定了谷胱甘肽过氧化物酶（GPX）的活性。
完全性葡萄胎患者的8 - OHdG/dG比值（2.8148 ± 0.81592）和MDA（10.8341 ± 4.64875 μmol）显著高于其他两组，而泛醇 - 10/泛醌 - 10比值（0.2107 ± 0.15675）和GPX活性（43.4606 ± 18.31694 mU/mI）则较低（p < 0.001）。
作者认为线粒体氧化和氧化性DNA损伤在完全性葡萄胎的发病机制中均起重要作用。因此，补充CoQ10可预防复发性妊娠滋养细胞疾病。
