Aksoy Ahmet, Kurnaz Senem Çengel
Department of Otorhinolaryngology, Faculty of Medicine, Nigde University, Fertek Mahallesi, 51000, Merkez/Nigde, Turkey.
Department of Otorhinolaryngology, Faculty of Medicine, Bahcesehir University, Istanbul, Turkey.
Eur Arch Otorhinolaryngol. 2019 Apr;276(4):1197-1204. doi: 10.1007/s00405-019-05328-5. Epub 2019 Feb 7.
The purpose of this study was to determine the oxidative states of head and neck squamous cell carcinoma (HNSCC) patients by measuring their plasma levels of malondialdehyde (MDA), an indicator of lipid peroxidation, 3-nitrotyrosine (3-NT), an indicator of protein oxidation, and the coenzyme Q10 (CoQ10), an important antioxidant, and compare them with healthy individuals.
The plasma MDA, 3-NT and CoQ10 levels of 35 patients and 20 healthy individuals were measured with the high-performance liquid chromatography (HPLC) method. By comparing the patients' smoking habits, stage of the disease, size of the primary tumor and the presence of lymph nodes and the values of healthy individuals, the oxidative stress load of HNSCC patients was determined.
The mean plasma MDA levels of carcinoma patients were two times higher than those of healthy individuals (p < 0.001). When the mean plasma 3-NT levels of patients and healthy individuals were compared, no significant difference was found (p > 0.05). The mean plasma CoQ10 level of patients was low when compared with healthy individuals; however, no significant difference was detected (p > 0.05). In addition, as the stage and tumor size increased in HNSCC patients, their non-enzymatic antioxidant levels significantly decreased (p < 0.05).
In HNSCC patients, lipo-oxidative damage increased while nitrosative stress did not change; however, antioxidant activity decreased which in turn increased both lipid peroxidation and oxidative stress. These findings support the contention that oxidative stress strongly reflects the health status of HNSCC patients.
本研究旨在通过测量头颈部鳞状细胞癌（HNSCC）患者血浆中丙二醛（MDA，脂质过氧化指标）、3-硝基酪氨酸（3-NT，蛋白质氧化指标）和辅酶Q10（CoQ10，一种重要的抗氧化剂）的水平来确定其氧化状态，并与健康个体进行比较。
采用高效液相色谱（HPLC）法测量35例患者和20名健康个体的血浆MDA、3-NT和CoQ10水平。通过比较患者的吸烟习惯、疾病分期、原发肿瘤大小、淋巴结情况以及健康个体的值，确定HNSCC患者的氧化应激负荷。
癌症患者的血浆MDA平均水平比健康个体高两倍（p < 0.001）。比较患者和健康个体的血浆3-NT平均水平时，未发现显著差异（p > 0.05）。与健康个体相比，患者的血浆CoQ10平均水平较低；然而，未检测到显著差异（p > 0.05）。此外，随着HNSCC患者分期和肿瘤大小增加，其非酶抗氧化剂水平显著降低（p < 0.05）。
在HNSCC患者中，脂氧化损伤增加而亚硝化应激未改变；然而，抗氧化活性降低，这反过来又增加了脂质过氧化和氧化应激。这些发现支持氧化应激强烈反映HNSCC患者健康状况这一观点。
