Lin R Y, Levin M, Nygren E N, Norman A, Lorenzana F G
Department of Medicine, Metropolitan Hospital, New York Medical College.
J Lab Clin Med. 1990 Oct;116(4):479-86.
Two different methods for evaluating platelet antibody were used to study 12 normal subjects and 24 patients consisting primarily of intravenous drug users (IVDUs) who were positive for human immunodeficiency virus (HIV). Total platelet-associated immunoglobulin G (IgG) and immunoglobulin M (IgM) were measured by enzyme-lined immunosorbent assay on platelet lysate, and platelet surface-associated IgG and IgM were measured by semiquantitative flow cytometry. IgG and IgM values showed significant correlations between the two measurement methods. Mean platelet surface IgG and total IgG were 3.6 and 4.3 times greater, respectively, in IVDUs than in controls, and platelet IgM was also significantly higher in IVDUs than in controls as measured by both techniques. Although mean platelet immunoglobulin levels were higher in the IVDUs with thrombocytopenia than in IVDUs with normal platelet counts, these differences did not achieve significance. These data show that platelet IgG and IgM levels are increased in IVDU-associated HIV infection and suggest that these increases are not confined to patients manifesting thrombocytopenia. The herein described platelet surface antibody and total platelet antibody measurements appear to be equally useful in studying this patient population. Specific details for generating platelet-associated immunofluorescence units are discussed.
采用两种不同的方法评估血小板抗体，对12名正常受试者和24名主要由静脉吸毒者（IVDUs）组成的患者进行研究，这些静脉吸毒者的人类免疫缺陷病毒（HIV）检测呈阳性。通过酶联免疫吸附测定法检测血小板裂解物上的总血小板相关免疫球蛋白G（IgG）和免疫球蛋白M（IgM），并通过半定量流式细胞术检测血小板表面相关IgG和IgM。IgG和IgM值在两种测量方法之间显示出显著相关性。IVDUs的平均血小板表面IgG和总IgG分别比对照组高3.6倍和4.3倍，并且通过两种技术测量，IVDUs的血小板IgM也显著高于对照组。虽然血小板减少的IVDUs的平均血小板免疫球蛋白水平高于血小板计数正常的IVDUs，但这些差异未达到显著水平。这些数据表明，IVDU相关的HIV感染中血小板IgG和IgM水平升高，提示这些升高并不局限于表现为血小板减少的患者。本文所述的血小板表面抗体和总血小板抗体测量在研究该患者群体中似乎同样有用。讨论了生成血小板相关免疫荧光单位的具体细节。
