[血小板相关IgG、IgM和C3的酶联免疫吸附测定及其临床应用]

[ELISA for platelet-associated IgG, IgM, and C3, and their clinical application].

作者信息

Enokido C, Kawaguchi R, Haruna S, Yonezawa M, Hikiji K, Ishigami T, Takahashi M, Nomura T

出版信息

Rinsho Byori. 1989 Aug;37(8):923-8.

Abstract

The platelet-associated IgG (PAIgG) has been reported to elevate in the patients with idiopathic thrombocytopenic purpura (ITP) and other autoimmune diseases. However, low PAIgG levels have been often recognized in thrombocytopenia. We speculated about the increasing of other platelet-associated proteins in those patients, and tried to determine platelet-associated IgM (PAIgM) and platelet-associated C3 (PAC3) using a high sensitive competitive micro-ELISA as well as PAIgG. Our results showed the specific elevation of PAIgM and PAC3 in thrombocytopenia as well as the PAIgG level (p less than 0.01). Further, the weak correlations among these levels were found (PAIgG/PAIgM: n = 7, correlation coefficient (r) = 0.55, PAIgG/PAC3: n = 73, r = 0.61, PAIgM/PAC3: n = 56, r = 0.39). We discussed on the possibility that the PAIgM and PAC3 also could be an indicator for the platelet injury and may cause the short platelet life span resulting thrombocytopenia as well as PAIgG.

摘要

据报道，特发性血小板减少性紫癜（ITP）患者及其他自身免疫性疾病患者的血小板相关IgG（PAIgG）水平会升高。然而，血小板减少症患者中经常出现PAIgG水平较低的情况。我们推测这些患者体内其他血小板相关蛋白会增加，并尝试使用高灵敏度竞争性微量酶联免疫吸附测定法（micro-ELISA）以及PAIgG来测定血小板相关IgM（PAIgM）和血小板相关C3（PAC3）。我们的结果显示，血小板减少症患者的PAIgM和PAC3以及PAIgG水平均有特异性升高（p小于0.01）。此外，还发现这些水平之间存在弱相关性（PAIgG/PAIgM：n = 7，相关系数（r）= 0.55；PAIgG/PAC3：n = 73，r = 0.61；PAIgM/PAC3：n = 56，r = 0.39）。我们讨论了PAIgM和PAC3也可能是血小板损伤指标的可能性，并且可能像PAIgG一样导致血小板寿命缩短，从而引起血小板减少症。