一种用于严重失血性休克的“处理后”模型：传统动物与无菌动物的比较。

A "treated" model for severe hemorrhagic shock: a comparison of conventional and germ-free animals.

作者信息

Flanagan J J, Rush B F, Murphy T F, Smith S, Machiedo G W, Hsieh J, Rosa D M, Heneghan J B

机构信息

Department of Surgery, UMD-New Jersey Medical School, Newark 07103.

出版信息

J Med. 1990;21(1-2):104-20.

PMID:2212865

Abstract

In an unanesthetized "treated" model of severe hemorrhagic shock, a bacteremia originating from the animal's enteric flora was demonstrated by finding radiolabeled Escherichia coli in the blood as early as 2 hr after the onset of shock. In 50 patients admitted to our trauma unit, the observation was similar, with 56% positive blood cultures, when their mean arterial blood pressure was 80 mmHg or less. The relationship of bacterial translocation and the high post-shock mortality in our conventional (CV) animal model is being evaluated in germfree (GF) Sprague-Dawley rats. Preliminary observations in 15 GF animals showed 80% survival at 24 hr post-shock, 54% at 48 hr, and 42% at 72 hr compared with those of CV animals in previous experiments with survival at similar times of 73%, 20% and 7%, respectively.

摘要

在未麻醉的严重失血性休克“治疗”模型中，早在休克发作后2小时，通过在血液中发现放射性标记的大肠杆菌，证实了源自动物肠道菌群的菌血症。在我们创伤科收治的50例患者中，当平均动脉血压为80 mmHg或更低时，观察结果相似，血培养阳性率为56%。我们正在无菌（GF）的斯普拉格-道利大鼠中评估传统（CV）动物模型中细菌易位与休克后高死亡率之间的关系。在15只GF动物中的初步观察显示，休克后24小时存活率为80%，48小时为54%，72小时为42%，而在之前的实验中，CV动物在相似时间的存活率分别为73%、20%和7%。