Dipartimento di Chimica, Università degli Studi di Torino , via P. Giuria 7, Torino 10125, Italy.
Chem Res Toxicol. 2012 Jan 13;25(1):74-82. doi: 10.1021/tx200255h. Epub 2011 Dec 14.
Two samples of highly pure multiwalled carbon nanotubes (MWCNTs) similar in hydrophobicity and surface reactivity were prepared with similar length, <5 μm, but markedly different diameter (9.4 vs 70 nm). The samples were compared for their cytotoxic activity, uptake, and ability to induce oxidative stress (ROS production and intracellular GSH depletion) in vitro in murine alveolar macrophages (MH-S). The in vivo toxicity was evaluated by measuring biochemical (LDH activity and total proteins) and cellular responses in bronchoalveolar lavage (BAL) after intratracheal instillation in rats. Both samples were internalized in MH-S cells. However, thin MWCNTs appeared significantly more toxic than the thicker ones, both in vitro and in vivo, when compared on a mass-dose basis. The data reported herein suggest that the nanotube diameter is an important parameter to be considered in the toxicological assessment of CNTs.
两种具有相似疏水性和表面反应性的高纯度多壁碳纳米管（MWCNTs）样品被制备出来，它们的长度相似，都小于 5μm，但直径明显不同（9.4nm 与 70nm）。对这两种样品的体外细胞毒性、摄取能力以及在体外诱导氧化应激（ROS 产生和细胞内 GSH 耗竭）的能力在鼠肺泡巨噬细胞（MH-S）中进行了比较。通过测量气管内滴注后大鼠支气管肺泡灌洗液（BAL）中的生化（LDH 活性和总蛋白）和细胞反应，评估了两种样品的体内毒性。两种样品都能被 MH-S 细胞内化。然而，当以质量剂量为基础进行比较时，与较厚的 MWCNTs 相比，薄 MWCNTs 在体外和体内均表现出明显更高的毒性。本文报道的数据表明，在 CNTs 的毒理学评估中，纳米管直径是一个需要考虑的重要参数。
