Istanbul Robert College, Istanbul, Turkey.
Int Wound J. 2012 Oct;9(5):478-87. doi: 10.1111/j.1742-481X.2011.00897.x. Epub 2011 Dec 1.
Delayed wound healing in elderly males is a complex process in which the factors responsible are not fully understood. This study investigated the hormonal, oxidative and angiogenic factors affecting wound healing in aged rats. Two groups consisting of eight healthy male Wistar Albino rats [young (30 ± 7 days) and aged (360 ± 30 days)], and a cutaneous incision wound healing model were used. Scar tissue samples from wounds on the 7th, 14th and 21st days of healing were evaluated for hydroxyproline and vascular endothelial growth factor content. Macrophage, lymphocyte, fibroblast and polymorphonuclear cell infiltration; collagen formation and vascularization were assessed by light and electron microscopy. The free oxygen radical content of the wounds was measured by a chemiluminescence method. Blood sample analysis showed that the hydroxyproline and total testosterone levels were significantly higher, and the oxygen radical content was significantly lower in young rats. Histopathological, immunohistochemical and ultrastructural evaluations revealed higher amounts of fibroblasts and collagen fibers, and more vascularization in young rats. These results are indicative of the delayed wound healing in aged rats. A combination of multiple factors including hormonal regulation, free oxygen radicals and impaired angiogenesis appears to be the cause of delayed cutaneous healing.
老年男性伤口愈合延迟是一个复杂的过程，其具体原因尚未完全阐明。本研究旨在探讨影响老年大鼠伤口愈合的激素、氧化和血管生成因素。研究使用了两组共 8 只健康雄性 Wistar 白化大鼠（年轻组，30±7 天；老年组，360±30 天），并建立了皮肤切口伤口愈合模型。在愈合第 7、14 和 21 天，对伤口的疤痕组织样本进行羟脯氨酸和血管内皮生长因子含量评估。通过光镜和电镜评估巨噬细胞、淋巴细胞、成纤维细胞和多形核细胞浸润、胶原蛋白形成和血管生成情况。通过化学发光法测量伤口的游离氧自由基含量。血液样本分析显示，年轻大鼠的羟脯氨酸和总睾酮水平显著升高，氧自由基含量显著降低。组织病理学、免疫组织化学和超微结构评估显示，年轻大鼠的成纤维细胞和胶原纤维较多，血管化程度更高。这些结果表明老年大鼠的伤口愈合延迟。多种因素的综合作用，包括激素调节、游离氧自由基和受损的血管生成，可能是导致皮肤愈合延迟的原因。
