Wang Yize, Wang Xianzhu, Zhou Dan, Xia Xin, Zhou Huimin, Wang Ying, Ke Huizhen
College of Textile and Clothing, XinJiang University, Wulumuqi 830046, China.
College of Fashion and Art Engineering, Minjiang University, Fuzhou, Fujian 350108, China.
ACS Omega. 2023 May 26;8(23):20323-20331. doi: 10.1021/acsomega.2c08180. eCollection 2023 Jun 13.
In recent years, medicinal plant extracts have received remarkable attention due to their wound-healing properties. In this study, polycaprolactone (PCL) electrospun nanofiber membranes incorporated with different concentrations of pomegranate peel extract (PPE) were prepared. The results of the SEM and FTIR experiments demonstrated that the morphology of nanofiber is smooth, fine, and bead-free, and the PPE was well introduced into the nanofiber membranes. Moreover, the outcomes of the mechanical property tests demonstrated that the nanofiber membrane made of PCL and loaded with PPE exhibited remarkable mechanical characteristics, indicating that it could fulfill the essential mechanical requisites for wound dressings. The findings of the in vitro drug release investigations indicated that PPE was instantly released within 20 h and subsequently released gradually over an extended period by the composite nanofiber membranes. Meanwhile, the DPPH radical scavenging test confirmed that the nanofiber membranes loaded with PPE exhibited significant antioxidant properties. Antimicrobial experiments showed higher PPE loading, and the nanofiber membranes showed higher antimicrobial activity against , and . The results of the cellular experiments showed that the composite nanofiber membranes were nontoxic and promoted the proliferation of L929 cells. In summary, electrospun nanofiber membranes loaded with PPE can be used as a wound dressing.
近年来，药用植物提取物因其伤口愈合特性而受到显著关注。在本研究中，制备了掺入不同浓度石榴皮提取物（PPE）的聚己内酯（PCL）电纺纳米纤维膜。扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）实验结果表明，纳米纤维形态光滑、精细且无珠粒，PPE被良好地引入到纳米纤维膜中。此外，力学性能测试结果表明，由PCL制成并负载PPE的纳米纤维膜表现出显著的力学特性，表明它能够满足伤口敷料的基本力学要求。体外药物释放研究结果表明，PPE在20小时内迅速释放，随后由复合纳米纤维膜在较长时间内逐渐释放。同时，二苯基苦味酰基自由基（DPPH）清除试验证实，负载PPE的纳米纤维膜具有显著的抗氧化性能。抗菌实验表明，PPE负载量越高，纳米纤维膜对 、 和 的抗菌活性越高。细胞实验结果表明，复合纳米纤维膜无毒，并促进L929细胞的增殖。综上所述，负载PPE的电纺纳米纤维膜可作为伤口敷料使用。
