State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Environ Sci Technol. 2012 Jan 17;46(2):795-801. doi: 10.1021/es2025998. Epub 2011 Dec 22.
A full-scale biosystem consisting of two anaerobic reactors (HA and BF1) and four aerobic ones (BF2-BF4 and OD) in succession and receiving antibiotic-bearing (mainly streptomycin) wastewater was used for studying the impacts of antibiotics on microbial community structures. Significant decreases of streptomycin (from 3955 ± 1910 to 23.1 ± 4.7 μg L(-1)) and COD(Cr) were observed along the treatment process. Cloning results show that the anaerobic reactors (HA and BF1) were dominated with Deltaproteobacteria (51%) mainly affiliated with sulfate-reducing bacteria (SRB), while the aerobic BF2 receiving streptomycin of 408.6 ± 59.7 μg L(-1) was dominated with Betaproteobacteria (34%), Deltaproteobacteria (31%) and Bacteroidetes (14%). Gammaproteobacteria (15.9-22.4%), Betaproteobacteria (10.0-20.3%), and Bacteroidetes (4.5-29.7%) became the major bacterial groups in aerobic BF3-OD receiving streptomycin of ≤83 ± 13 μg L(-1). Archaea affiliated with Methanomethylovorans hollandica-like methylotroph was abundant in HA and BF1 (archaea/bacteria, 0.54-0.40; based on specific gene copy number), suggesting the coexistence of SRB and methanogens in degrading pollutants. Fungi were abundant (fungi/bacteria, 0.15; based on specific gene copy number) with the dominance of Ascomycota (clone ratio of Ascomycota/eukarya, 25.5%) in BF2, suggesting that fungi could be an important player in pollutant removal under high levels of antibiotics. This study demonstrates that under high antibiotic levels, wastewater treatment communities may maintain system stability through adjusting bacterial, archaeal, and eukaryal compositions.
采用由两个厌氧反应器（HA 和 BF1）和四个好氧反应器（BF2-BF4 和 OD）连续组成的全尺寸生物系统，接收含有抗生素（主要是链霉素）的废水，用于研究抗生素对微生物群落结构的影响。沿处理过程观察到链霉素（从 3955 ± 1910 降至 23.1 ± 4.7 μg L(-1)) 和 COD(Cr) 的显著减少。克隆结果表明，厌氧反应器（HA 和 BF1）以δ变形菌（51%）为主，主要与硫酸盐还原菌（SRB）有关，而接收 408.6 ± 59.7 μg L(-1) 链霉素的好氧 BF2 则以β变形菌（34%）、δ变形菌（31%）和拟杆菌门（14%）为主。γ变形菌（15.9-22.4%）、β变形菌（10.0-20.3%）和拟杆菌门（4.5-29.7%）成为接收≤83 ± 13 μg L(-1) 链霉素的好氧 BF3-OD 的主要细菌类群。HA 和 BF1 中丰富的与 Methanomethylovorans hollandica-like methylotroph 相关的古菌（古菌/细菌，0.54-0.40；基于特定基因拷贝数）表明，在降解污染物时，SRB 和产甲烷菌共存。真菌（真菌/细菌，0.15；基于特定基因拷贝数）丰富，其中子囊菌门（子囊菌门/真核生物，25.5%）占优势，表明真菌可能是在高抗生素水平下去除污染物的重要参与者。本研究表明，在高抗生素水平下，废水处理群落可能通过调整细菌、古菌和真核生物的组成来维持系统稳定性。
