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通过 Fmoc-Phe-Phe 的冷冻凝胶化形成大孔自组装水凝胶。

Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe.

机构信息

Department of Biotechnology, Lund University, P.O. Box 124, 22 100 Lund, Sweden.

出版信息

J Colloid Interface Sci. 2012 Feb 15;368(1):226-30. doi: 10.1016/j.jcis.2011.11.006. Epub 2011 Nov 12.

Abstract

In this study, it was found that macroporous hydrogels were formed when self-assembly of fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) peptides was induced using glucono-δ-lactone (GdL) in apparently frozen samples. Formed cryogels exhibited a heterogeneous structure with pore walls of densely packed fibres of assembled dipeptides and pores in the range 10-100 μm. Hydrogels formed from the same composition above the freezing point exhibited a homogenous structure without any apparent porosity. The formed gels were characterised using microscopy techniques, CD-spectroscopy and stress sweeps. The cryogels exhibited less mechanical strength than the hydrogels that might be due to the heterogeneous structure of the former. It appeared that the self-assembled peptide both in the cryo- and hydrogel maintained the β-sheet structure commonly attributed to these.

摘要

在这项研究中,发现当使用葡萄糖酸-δ-内酯(GdL)诱导芴甲氧羰基-9-(二苯基)-(Fmoc)-二苯丙氨酸(Phe-Phe)肽的自组装时,在明显冷冻的样品中形成了大孔水凝胶。形成的冷冻凝胶表现出不均匀的结构,组装的二肽纤维的孔壁密集,孔径在 10-100μm 范围内。在冰点以上由相同组成形成的水凝胶表现出均匀的结构,没有任何明显的孔隙率。使用显微镜技术、CD 光谱和应力扫描对形成的凝胶进行了表征。冷冻凝胶的机械强度低于水凝胶,这可能是由于前者的不均匀结构所致。似乎在冷冻和水凝胶中的自组装肽都保持了通常归因于这些的β-折叠结构。

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