Institute of Biomedical Engineering & Department of Chemistry, Imperial College London, South Kensington Campus, Exhibition Road, London, SW7 2AZ, UK.
Lab Chip. 2012 Jan 21;12(2):348-52. doi: 10.1039/c1lc20885c. Epub 2011 Nov 30.
In this work we demonstrate a novel microfluidic based platform to investigate the performance of 3D out-of-plane microspike array based glucose and lactate biosensors. The microspike array was bonded with a glass slide and modified with glucose oxidase or lactate oxidase using covalent coupling chemistry. An epoxy-polyurethane based membrane was used to extend the linear working range (from 0 to 25 mM of substrate) of these biosensors. Both lactate and glucose sensors performed well in the clinically relevant substrate concentration range. Glucose microspikes were further investigated with respect to the effects of substrate transfer by incorporation into a microfluidic system. Data from the microfluidic system revealed that the sensor response is mainly dependent on enzyme kinetics rather than membrane permeability to glucose. The robustness of the sensors was demonstrated by its consistency in performance extending over 48 h.
在这项工作中，我们展示了一种基于微流控的新型平台，用于研究基于 3D 离面微刺阵列的葡萄糖和乳酸生物传感器的性能。微刺阵列通过共价偶联化学与玻璃载片键合，并使用葡萄糖氧化酶或乳酸氧化酶进行修饰。基于环氧树脂-聚亚安酯的膜用于扩展这些生物传感器的线性工作范围（从 0 到 25mM 的基质）。乳酸和葡萄糖传感器在临床相关的基质浓度范围内均表现良好。进一步将葡萄糖微刺整合到微流控系统中，研究了基质传递对其的影响。微流控系统的数据表明，传感器的响应主要取决于酶动力学，而不是葡萄糖通过膜的渗透率。传感器的稳健性通过其在 48 小时内性能的一致性得到了证明。
