Analysis of herpes simplex virus low molecular-weight native proteins by polyacrylamide gel electrofocusing.

This communication has demonstrated the potential application of gel electrofocusing for the isolation and further characterization of HSV-2 native proteins. At least eight major HSV-2 specific proteins with characteristic isoelectric points could be reproducibly resolved by gel isoelectric focusing. Their isoelectric points ranged from 5.0 to 7.5 and analysis by two-dimensional electrophoresis on SDS gels indicated that their molecular weights range from 11,000 to 49,000.