Wolf H, Roizman B
IARC Sci Publ (1971). 1978(24 Pt 1):327-36.
The polypeptides specified by herpes simplex viruses types 1 and 2 form at least three groups designated as alpha, beta and gamma. The structural polypeptides are largely contained in the gamma polypeptide group. In this study, we investigated the transition from beta to gamma polypeptide synthesis. Our data show the following: (i) gamma polypeptide synthesis in the presence of inhibitors of DNA synthesis in the presence of inhibitors of DNA synthesis is multiplicity-dependent. Some gamma polypeptides are not detectable in cells infected with 1 PFU per cell form a significant fraction of viral polypeptides in cells infected with 25--500 PFU per cell. (ii) The mRNAs specifying these polypeptides have a relatively short half-life as measured by the relative rate of decay of gamma polypeptide synthesis in infected cells following exposure to actinomycin D. Our data thus suggest that: (i) the transition from beta to gamma polypeptide synthesis does not require the synthesis of viral DNA; and (ii) the rate of synthesis of viral structural (gamma) polypeptides is linked to the size of the viral DNA pool as a consequence of a relatively short-lived mRNA.
1型和2型单纯疱疹病毒所特有的多肽至少形成三组，分别命名为α、β和γ。结构多肽主要包含在γ多肽组中。在本研究中，我们调查了从β多肽合成到γ多肽合成的转变。我们的数据显示如下：（i）在存在DNA合成抑制剂的情况下，γ多肽合成具有多重依赖性。在每细胞感染1个噬斑形成单位（PFU）的细胞中，一些γ多肽无法检测到，而在每细胞感染25 - 500个PFU的细胞中，它们占病毒多肽的很大一部分。（ii）通过放线菌素D处理后感染细胞中γ多肽合成的相对衰减率来衡量，编码这些多肽的mRNA半衰期相对较短。因此，我们的数据表明：（i）从β多肽合成到γ多肽合成的转变不需要病毒DNA的合成；（ii）由于mRNA寿命相对较短，病毒结构（γ）多肽的合成速率与病毒DNA库的大小相关。
