Pedersen M, Talley-Brown S, Millette R L
J Virol. 1981 May;38(2):712-9. doi: 10.1128/JVI.38.2.712-719.1981.
Arabinosyladenine, an established antiherpetic drug, was used to block herpes simplex virus type 1 DNA synthesis quantitatively in infected xeroderma pigmentosum cells. Kinetic analyses of viral polypeptides synthesized in the presence and absence of this drug revealed that there were at least six distinct kinetic classes of polypeptides. These differed in time of appearance after infection, time of maximum rate of synthesis, kinetics of turnoff, and sensitivity to arabinosyladenine. This study showed that arabinosyladenine had the following three main effects on herpes simplex virus type 1 gene expression. (i) The turnon of immediate early and delayed early polypeptides (kinetic classes 1 and 2) was retarded. (ii) The turnoff of early (immediate early and delayed early) polypeptides (classes 1 through 3) was delayed. (iii) The synthesis of late polypeptides (class 4 through 6) was inhibited by arabinosyladenine, with class 6 severely (80 to 90%) inhibited. The kinetic data presented here, along with the findings of other workers on the effects of inhibition of viral DNA synthesis, suggest that viral DNA replication is required for optimum synthesis of late viral polypeptides.
阿糖腺苷是一种已获认可的抗疱疹药物，被用于定量阻断单纯疱疹病毒1型（HSV-1）在感染的着色性干皮病细胞中的DNA合成。对在有或没有这种药物存在的情况下合成的病毒多肽进行动力学分析发现，至少有六种不同的动力学类别。这些类别在感染后的出现时间、合成最大速率的时间、关闭动力学以及对阿糖腺苷的敏感性方面存在差异。这项研究表明，阿糖腺苷对单纯疱疹病毒1型基因表达有以下三个主要影响。（i）立即早期和延迟早期多肽（动力学类别1和2）的开启受到延迟。（ii）早期（立即早期和延迟早期）多肽（类别1至3）的关闭被延迟。（iii）晚期多肽（类别4至6）的合成被阿糖腺苷抑制，其中类别6受到严重抑制（80%至90%）。这里呈现的动力学数据，以及其他研究人员关于抑制病毒DNA合成效果的发现，表明病毒DNA复制是晚期病毒多肽最佳合成所必需的。
