Gualdoni Sara, Baron Michael, Lakowski Jörn, Decembrini Sarah, Pearson Rachel A, Ali Robin R, Sowden Jane C
Developmental Biology Unit, UCL Institute of Child Health, University College London, London, United Kingdom.
Curr Protoc Stem Cell Biol. 2011 Dec;Chapter 1:Unit 1H.4. doi: 10.1002/9780470151808.sc01h04s19.
The protocols described in this unit provide detailed information on how to isolate and expand, in culture, ciliary epithelial cells (CECs), previously identified as retinal stem cells, from the adult mouse eye, and embryonic retinal progenitor cells (RPCs) from the developing retina. CECs are initially cultured in floating conditions as neurospheres and then expanded in monolayer cultures. RPCs are cultured in floating conditions. Detailed protocols for retinal differentiation, as well as exogenous gene expression using lentivirus are also described.
