缺铁性贫血绝经前女性的外周血淋巴细胞亚群计数

Peripheral Blood Lymphocyte Subset Counts in Pre-menopausal Women with Iron-Deficiency Anaemia.

作者信息

Reza Keramati Mohammad, Sadeghian Mohammad Hadi, Ayatollahi Hossein, Mahmoudi Mahmoud, Khajedaluea Mohammad, Tavasolian Houman, Borzouei Anahita

机构信息

Neonatal Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Malays J Med Sci. 2011 Jan;18(1):38-44.

PMID:22135572

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3216203/

Abstract

BACKGROUND

Iron-deficiency anaemia (IDA) is a major worldwide public health problem. Children and women of reproductive age are especially vulnerable to IDA, and it has been reported that these patients are more prone to infection. This study was done to evaluate alteration of lymphocyte subgroups in IDA.

METHODS

In this prospective study, we investigated lymphocyte subsets in pre-menopausal women with iron-deficiency anaemia; 50 normal subjects and 50 IDA (hypochromic microcytic) cases were enrolled. Experimental and control anticoagulated blood samples were evaluated using flow cytometry to determine the absolute and relative numbers of various lymphocyte subgroups. Finally, the results of the patient and control groups were compared.

RESULTS

Mean (SD) absolute counts of lymphocytes, CD3+ cells, CD3+/CD4+ subsets (T helper) and CD3+/CD8+ subsets (T cytotoxic) in the patient group were 2.08 (0.65) x 109/L, 1.53 (0.53) x 10(9)/L, 0.87 (0.28) x 109/L, and 0.51 (0.24) x 10(9)/L, respectively. The results showed significant differences between case and control groups in mean absolute counts of lymphocytes (P = 0.014), T lymphocytes (P = 0.009), helper T cells (P = 0.004), and cytotoxic T cells (P = 0.043).

CONCLUSION

This study showed that absolute counts of peripheral blood T lymphocytes as a marker of cell-mediated immunity may be decreased in pre-menopausal women with iron-deficiency anaemia, and that these patients may be more prone to infection.

摘要

背景

缺铁性贫血（IDA）是一个全球性的主要公共卫生问题。儿童和育龄妇女尤其易患缺铁性贫血，并且据报道这些患者更容易感染。本研究旨在评估缺铁性贫血患者淋巴细胞亚群的变化。

方法

在这项前瞻性研究中，我们调查了缺铁性贫血的绝经前女性的淋巴细胞亚群；纳入了50名正常受试者和50例缺铁性贫血（低色素小细胞性）病例。使用流式细胞术评估实验和对照抗凝血液样本，以确定各种淋巴细胞亚群的绝对数量和相对数量。最后，比较患者组和对照组的结果。

结果

患者组淋巴细胞、CD3 +细胞、CD3 + / CD4 +亚群（辅助性T细胞）和CD3 + / CD8 +亚群（细胞毒性T细胞）的平均（标准差）绝对计数分别为2.08（0.65）×10⁹/L、1.53（0.53）×10⁹/L、0.87（0.28）×10⁹/L和0.51（0.24）×10⁹/L。结果显示，病例组和对照组在淋巴细胞平均绝对计数（P = 0.014）、T淋巴细胞（P = 0.009）、辅助性T细胞（P = 0.004）和细胞毒性T细胞（P = 0.043）方面存在显著差异。