Aly Sanaa S, Fayed Hanan M, Ismail Ahlam M, Abdel Hakeem Gehan L
Clinical and chemical pathology Department, Faculty of Medicine, South Valley University, Quena, Egypt.
Peditretic Department, Faculty of Medicine, South Valley University, Qena, Egypt.
BMC Pediatr. 2018 Feb 12;18(1):49. doi: 10.1186/s12887-018-0990-5.
Iron plays an important role in body defense and essential for normal immune system development where its deficiency may result in an inadequate immune response. We aimed to assess the lymphocyte subsets in childhood iron deficiency anemia (IDA) with their laboratory correlations.
Fifty IDA (< 18 years) and 25 age and sex-matched healthy children were enrolled and a complete history was obtained and clinical examination was performed. Complete blood count, serum iron, total iron binding capacity and serum ferritin, were performed. Flow cytometric determination of peripheral blood CD3+, CD4+, CD8+ T-lymphocytes and CD19+ B-lymphocytes and CD4/CD8 ratio were done.
Patients had significantly lower hemoglobin, Serum iron, ferritin levels and higher lymphocytic count in patients compared with controls (p = 0.001, 0.03, 0.001, 0.001 respectively). CD3 count and percentage were significantly lower in IDA patients compared to controls (p = 0.007 and 0.005 respectively). There was a Significant reduction in the CD4 count, percentage and CD4/CD8 ratio in patients compared with controls (p = 0.001, 0.001 and 0.005 respectively) while there was no significant difference regarding CD8 count and percentage. No significant difference between the two studied groups regarding either CD19 count or percentage (p = 0.28 and 0.18 respectively) were found.
IDA is associated with impaired cell-mediated immune response specifically T-cell mediated immunity.
铁在机体防御中发挥重要作用，是正常免疫系统发育所必需的，缺铁可能导致免疫反应不足。我们旨在评估儿童缺铁性贫血（IDA）患者的淋巴细胞亚群及其实验室相关性。
纳入50例IDA患儿（<18岁）和25例年龄及性别匹配的健康儿童，获取完整病史并进行临床检查。检测全血细胞计数、血清铁、总铁结合力和血清铁蛋白。采用流式细胞术测定外周血CD3⁺、CD4⁺、CD8⁺T淋巴细胞、CD19⁺B淋巴细胞及CD4/CD8比值。
与对照组相比，患者的血红蛋白、血清铁、铁蛋白水平显著降低，淋巴细胞计数升高（p分别为0.001、0.03、0.001、0.001）。IDA患者的CD3计数和百分比显著低于对照组（p分别为0.007和0.005）。与对照组相比，患者的CD4计数、百分比及CD4/CD8比值显著降低（p分别为0.001、0.001和0.005），而CD8计数和百分比无显著差异。两组在CD19计数或百分比方面均无显著差异（p分别为0.28和0.18）。
IDA与细胞介导的免疫反应受损有关，尤其是T细胞介导的免疫。
