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连续传代的1型单纯疱疹病毒（贾斯汀株）中所含缺陷基因组的结构与起源

Structure and origin of defective genomes contained in serially passaged herpes simplex virus type 1 (Justin).

作者信息

Locker H, Frenkel N

出版信息

J Virol. 1979 Mar;29(3):1065-77. doi: 10.1128/JVI.29.3.1065-1077.1979.

DOI:10.1128/JVI.29.3.1065-1077.1979
PMID:221666
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC353267/
Abstract

Restriction enzyme and hybridization analyses have revealed that high-density DNA prepared from passage 15 of serially passaged herpes simplex virus type 1 (Justin) contains three major classes of modified viral DNA molecules, each composed of distinct but closely related types of repeate units. The DNA sequences within the three types of repeat units are colinear with the DNA sequences located at the right end (between coordinates 0.94 and 1.0) of the parental herpes simplex virus type 1 genome. Thus, the three types of repeat units each contain the entire repeat sequence (ac) (which brackets the unique sequences of the small [S] component of herpes simplex virus type 1 DNA) and differ only with respect to the amount of unique S sequences which they contain. The three classes of high-density DNA molecules were found to be stably propagated between passages 6 and 15 of this series.

摘要

限制性内切酶和杂交分析表明，从1型单纯疱疹病毒（贾斯汀株）连续传代15代所制备的高密度DNA含有三类主要的修饰病毒DNA分子，每一类都由不同但密切相关的重复单元类型组成。这三种重复单元类型中的DNA序列与亲本1型单纯疱疹病毒基因组右端（坐标0.94至1.0之间）的DNA序列共线。因此，这三种重复单元类型各自都包含完整的重复序列（ac）（该序列包围着1型单纯疱疹病毒DNA小[S]组分的独特序列），并且仅在所包含的独特S序列的量方面有所不同。发现这三类高密度DNA分子在该系列传代的第6代至第15代之间稳定传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/a4b706ca98af/jvirol00183-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/275fcbc6b598/jvirol00183-0247-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/275fcbc6b598/jvirol00183-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/9dc22b46443b/jvirol00183-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/6c8127f58575/jvirol00183-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/956dc8c7cc5b/jvirol00183-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/ea835e85172e/jvirol00183-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/89404267a792/jvirol00183-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e156/353267/a4b706ca98af/jvirol00183-0254-a.jpg

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An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction.一种由噬菌体λ诱导产生的核酸外切酶。II. 酶促反应的性质。
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