National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
J Proteome Res. 2012 Jan 1;11(1):372-85. doi: 10.1021/pr200701y. Epub 2011 Dec 13.
Flooding injury is a major problem in soybean cultivation. A proteomics approach was used to clarify the occurrence of changes in protein expression level and phosphorylation in soybeans under flooding stress. Two-day-old seedlings were flooded for 1 day, proteins were extracted from root tips of the seedlings and digested with trypsin, and their expression levels and phosphorylation states were compared to those of untreated controls using mass spectrometry-based proteomics techniques. Phosphoproteins were enriched using a phosphoprotein purification column prior to digestion and mass spectrometry. The expression of proteins involved in energy production increased as a result of flooding, while expression of proteins involved in protein folding and cell structure maintenance decreased. Flooding induced changes of phosphorylation status of proteins involved in energy generation, protein synthesis and cell structure maintenance. The response to flooding stress may be regulated by both modulation of protein expression and phosphorylation state. Energy-demanding and production-related metabolic pathways may be particularly subject to regulation by changes in protein phosphorylation during flooding.
flooding 损伤是大豆栽培中的一个主要问题。采用蛋白质组学方法来阐明在水淹胁迫下大豆中蛋白质表达水平和磷酸化的变化的发生。将两天大的幼苗水淹 1 天，从幼苗根尖提取蛋白质，并用胰蛋白酶消化，并用基于质谱的蛋白质组学技术比较未处理对照的蛋白质表达水平和磷酸化状态。在消化和质谱之前，用磷酸化蛋白纯化柱富集磷酸化蛋白。水淹导致与能量产生有关的蛋白质的表达增加，而与蛋白质折叠和细胞结构维持有关的蛋白质的表达减少。水淹诱导与能量产生、蛋白质合成和细胞结构维持有关的蛋白质磷酸化状态的变化。对水淹胁迫的反应可能受到蛋白质表达和磷酸化状态的调节。在水淹期间，能量需求和生产相关的代谢途径可能特别受到蛋白质磷酸化变化的调节。
