Sahoo Dipak Kumar, Hegde Chinmay, Bhattacharyya Madan K
Department of Agronomy, Iowa State University, Ames, IA, United States.
Department of Electrical and Computer Engineering, Iowa State University, Ames, IA, United States.
Front Plant Sci. 2023 Jan 12;13:1094462. doi: 10.3389/fpls.2022.1094462. eCollection 2022.
Cold stress adversely affects the growth and development of plants and limits the geographical distribution of many plant species. Accumulation of spontaneous mutations shapes the adaptation of plant species to diverse climatic conditions.
The genome-wide association study of the phenotypic variation gathered by a newly designed phenomic platform with the over six millions single nucleotide polymorphic (SNP) loci distributed across the genomes of 417 Arabidopsis natural variants collected from various geographical regions revealed 33 candidate cold responsive genes.
Investigation of at least two independent insertion mutants for 29 genes identified 16 chilling tolerance genes governing diverse genetic mechanisms. Five of these genes encode novel leucine-rich repeat domain-containing proteins including three nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins. Among the 16 identified chilling tolerance genes, and are the only two chilling tolerance genes identified earlier.
The 12.5% overlap between the genes identified in this genome-wide association study (GWAS) of natural variants with those discovered previously through forward and reverse genetic approaches suggests that chilling tolerance is a complex physiological process governed by a large number of genetic mechanisms.
低温胁迫对植物的生长发育产生不利影响，并限制了许多植物物种的地理分布。自发突变的积累塑造了植物物种对不同气候条件的适应性。
通过一个新设计的表型组学平台收集了表型变异，并对来自不同地理区域的417个拟南芥自然变体基因组中分布的超过600万个单核苷酸多态性（SNP）位点进行全基因组关联研究，发现了33个候选冷响应基因。
对29个基因的至少两个独立插入突变体进行研究，确定了16个控制不同遗传机制的耐冷基因。其中5个基因编码含新型富含亮氨酸重复结构域的蛋白质，包括3个核苷酸结合位点-富含亮氨酸重复序列（NBS-LRR）蛋白。在鉴定出的16个耐冷基因中，[此处原文缺失相关基因信息]和[此处原文缺失相关基因信息]是之前仅有的两个被鉴定出的耐冷基因。
在这项自然变体全基因组关联研究（GWAS）中鉴定出的基因与之前通过正向和反向遗传方法发现的基因之间有12.5%的重叠，这表明耐冷性是一个由大量遗传机制控制的复杂生理过程。
