Department of Pathology, Albert Einstein College of Medicine, Jack and Pearl Resnick Campus, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Int J Parasitol. 2012 Jan;42(1):33-7. doi: 10.1016/j.ijpara.2011.11.002. Epub 2011 Nov 22.
Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.
由于缺乏快速、简单和可逆的遗传工具来调节基因和相应蛋白质的表达，因此在克氏锥虫中分析基因功能受到限制。我们设计了一种改良的 pTREX 载体，该载体使用配体控制的不稳定化结构域（ddFKBP）与目标基因/蛋白质的 N 端融合。该载体允许在不同的克氏锥虫生命周期阶段快速和可逆地表达蛋白质，并有效地分析蛋白质的功能。
