Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, United States.
Biochem Biophys Res Commun. 2012 Jan 6;417(1):11-6. doi: 10.1016/j.bbrc.2011.11.058. Epub 2011 Nov 22.
Ever since the technique of coaxing ordinary skin cells into becoming pluripotent stem cells (iPSCs) has been developed, which have the potential to become any cell or tissue in the body, efforts were made to improve the approach because some major challenges. Increasing evidence suggests that several microRNAs (miRNAs) are involved in early embryonic development and embryonic stem cell formation, known as embryonic stem cell (ESC)-specific miRNAs, particularly the miR-302 family. We summarized here a novel approach to generate iPSCs by using miR-302 and its related miRNAs such as miR-367. The development of this miR-302/367-mediated iPSC (termed mirPSC) may provide tools to deal with the obstacles facing some current iPSC reprogramming methods. The mechanism by which miR-302/367 induce iPSC reprogramming is proposed.
自从诱导普通皮肤细胞成为多能干细胞（iPS 细胞）的技术被开发出来以来，这些细胞具有成为体内任何细胞或组织的潜力，因此人们努力改进该方法，因为存在一些重大挑战。越来越多的证据表明，几种 microRNAs（miRNAs）参与早期胚胎发育和胚胎干细胞形成，称为胚胎干细胞（ESC）特异性 miRNAs，特别是 miR-302 家族。在这里，我们总结了一种通过使用 miR-302 及其相关 miRNAs（如 miR-367）生成 iPS 细胞的新方法。这种 miR-302/367 介导的 iPSC（称为 mirPSC）的发展可能为解决一些当前 iPSC 重编程方法所面临的障碍提供工具。提出了 miR-302/367 诱导 iPSC 重编程的机制。
