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本文引用的文献

1
REFMAC5 for the refinement of macromolecular crystal structures.用于大分子晶体结构精修的REFMAC5
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67. doi: 10.1107/S0907444911001314. Epub 2011 Mar 18.
2
Molecular replacement with MOLREP.使用MOLREP进行分子置换。
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5. doi: 10.1107/S0907444909042589. Epub 2009 Dec 21.
3
MolProbity: all-atom structure validation for macromolecular crystallography.MolProbity：用于大分子晶体学的全原子结构验证
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. doi: 10.1107/S0907444909042073. Epub 2009 Dec 21.
4
Classification of 'Anaerocellum thermophilum' strain DSM 6725 as Caldicellulosiruptor bescii sp. nov.将“嗜热厌氧球菌”DSM 6725 菌株分类为 Caldicellulosiruptor bescii sp. nov.
Int J Syst Evol Microbiol. 2010 Sep;60(Pt 9):2011-2015. doi: 10.1099/ijs.0.017731-0. Epub 2009 Oct 2.
5
Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions.二级结构匹配（SSM），一种用于三维蛋白质结构快速比对的新工具。
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2256-68. doi: 10.1107/S0907444904026460. Epub 2004 Nov 26.
6
Coot: model-building tools for molecular graphics.Coot：分子图形的模型构建工具。
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. doi: 10.1107/S0907444904019158. Epub 2004 Nov 26.
7
Pectate lyases, cell wall degradation and fruit softening.果胶酸裂解酶、细胞壁降解与果实软化
J Exp Bot. 2002 Oct;53(377):2115-9. doi: 10.1093/jxb/erf089.
8
The first structure of pectate lyase belonging to polysaccharide lyase family 3.属于多糖裂解酶家族3的果胶酸裂解酶的首个结构。
Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1786-92. doi: 10.1107/s0907444901014482. Epub 2001 Nov 21.
9
Pectin: cell biology and prospects for functional analysis.果胶：细胞生物学与功能分析前景
Plant Mol Biol. 2001 Sep;47(1-2):9-27.

嗜热栖热放线菌3型果胶裂解酶催化模块的1.5埃分辨率X射线结构。

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase.

作者信息

Alahuhta Markus, Chandrayan Puja, Kataeva Irina, Adams Michael W W, Himmel Michael E, Lunin Vladimir V

机构信息

BioSciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1498-500. doi: 10.1107/S1744309111038449. Epub 2011 Nov 25.

DOI:10.1107/S1744309111038449
PMID:22139151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3232124/
Abstract

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R(free) of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 Å and a sequence identity of 53%. This structural similarity is significant considering that C. bescii is a hyperthermophile and Bacillus sp. is a mesophile.

摘要

报道了嗜热栖热放线菌3型果胶酸裂解酶催化模块的1.5埃分辨率X射线结构（蛋白质数据库条目3t9g）。所得结构的R因子精修至0.143，自由R值为0.178。结构分析表明，该新结构与先前解析的芽孢杆菌属菌株KSM-P15的3型果胶酸裂解酶结构非常相似（蛋白质数据库条目1ee6），均方根偏差为0.93埃，序列同一性为53%。考虑到嗜热栖热放线菌是嗜热菌，而芽孢杆菌属是嗜温菌，这种结构相似性意义重大。