嗜热栖热放线菌3型果胶裂解酶催化模块的1.5埃分辨率X射线结构。
A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase.
作者信息
Alahuhta Markus, Chandrayan Puja, Kataeva Irina, Adams Michael W W, Himmel Michael E, Lunin Vladimir V
机构信息
BioSciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1498-500. doi: 10.1107/S1744309111038449. Epub 2011 Nov 25.
Abstract
A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R(free) of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 Å and a sequence identity of 53%. This structural similarity is significant considering that C. bescii is a hyperthermophile and Bacillus sp. is a mesophile.
摘要
报道了嗜热栖热放线菌3型果胶酸裂解酶催化模块的1.5埃分辨率X射线结构（蛋白质数据库条目3t9g）。所得结构的R因子精修至0.143，自由R值为0.178。结构分析表明，该新结构与先前解析的芽孢杆菌属菌株KSM-P15的3型果胶酸裂解酶结构非常相似（蛋白质数据库条目1ee6），均方根偏差为0.93埃，序列同一性为53%。考虑到嗜热栖热放线菌是嗜热菌，而芽孢杆菌属是嗜温菌，这种结构相似性意义重大。
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